Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOA1 / C4orf14 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108907-100
Catalog Number: LS-G108907
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C4orf14(NOA1) (NM_032313), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C4orf14 | HNOA1 | MAtNOS1 | Nitric oxide associated-1 | NOA1 | HAtNOS1 | Nitric oxide associated 1
- Origin Species
- Human
- NCBI GenBank
- NM_032313
- SwissProt
- Q8NC60
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPARLPFRL LSLFLRGSAP TAARHGLREP LLERRCAAAS SFQHSSSLGR ELPYDPVDTE61GFGEGGDMQE RFLFPEYILD PEPQPTREKQ LQELQQQQEE EERQRQQRRE ERRQQNLRAR121SREHPVVGHP DPALPPSGVN CSGCGAELHC QDAGVPGYLP REKFLRTAEA DGGLARTVCQ181RCWLLSHHRR ALRLQVSREQ YLELVSAALR RPGPSLVLYM VDLLDLPDAL LPDLPALVGP241KQLIVLGNKV DLLPQDAPGY RQRLRERLWE DCARAGLLLA PGHQGPQRPV KDEPQDGENP301NPPNWSRTVV RDVRLISAKT GYGVEELISA LQRSWRYRGD VYLVGATNAG KSTLFNTLLE361SDYCTAKGSE AIDRATISPW PGTTLNLLKF PICNPTPYRM FKRHQRLKKD STQAEEDLSE421QEQNQLNVLK KHGYVVGRVG RTFLYSEEQK DNIPFEFDAD SLAFDMENDP VMGTHKSTKQ481VELTAQDVKD AHWFYDTPGI TKENCILNLL TEKEVNIVLP TQSIVPRTFV LKPGMVLFLG541AIGRIDFLQG NQSAWFTVVA SNILPVHITS LDRADALYQK HAGHTLLQIP MGGKERMAGF601PPLVAEDIML KEGLGASEAV ADIKFSSAGW VSVTPNFKDR LHLRGYTPEG TVLTVRPPLL661PYIVNIKGQR IKKSVAYKTK KPPSLMYNVR KKKGKINVTR TRPLEQKLIS EEDLAANDIL721DYKDDDDKV
729 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.