Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NNA1 / AGTPBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113380-100
Catalog Number: LS-G113380
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGTPBP1 (NM_015239), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP/GTP binding protein 1 | ATP/GTP-binding protein 1 | Carboxypeptidase-tubulin | Cytosolic carboxypeptidase 1 | AGTPBP1 | KIAA1035 | NNA1 | Tyrosine carboxypeptidase | Ccp1 | Soluble carboxypeptidase | Tubulinyl-Tyr carboxypeptidase
- Origin Species
- Human
- NCBI GenBank
- NM_015239
- SwissProt
- Q9UPW5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSKLKVIPEK SLTNNSRIVG LLAQLEKINA EPSESDTARY VTSKILHLAQ SQEKTRREMT61AKGSTGMEIL LSTLENTKDL QTTLNILSIL VELVSAGGGR RVSFLVTKGG SQILLQLLMN121ASKESPPHED LMVQIHSILA KIGPKDKKFG VKARINGALN ITLNLVKQNL QNHRLVLPCL181QLLRVYSANS VNSVSLGKNG VVELMFKIIG PFSKKNSSLI KVALDTLAAL LKSKTNARRA241VDRGYVQVLL TIYVDWHRHD NRHRNMLIRK GILQSLKSVT NIKLGRKAFI DANGMKILYN301TSQLPVIPVT GPVAQLYSLP PEVDDVVDES DDNDDIDVEA ENETENEDDL DQNFKNDDIE361TDINKLKPQQ EPGRTIEDLK MYEHLFPELV DDFQDYDLIS KEPKPFVFEG KVRGPIVVPT421AGEETSGNSG NLRKVVMKEN ISSKGDEGEK KSTFMDLAKE DIKDNDRTLQ QQPGDQNRTI481SSVHGLNNDI VKALDRITLQ NIPSQTAPGF TAEMKKDCSL PLTVLTCAKA CPHMATCGNV541LFEGRTVQLG KLCCTGVETE DDEDTESNSS VEQASVEVPD GPTLHDPDLY IEIVKNTKSV601PEYSEVAYPD YFGHIPPPFK EPILERPYGV QRTKIAQDIE RLIHQSDIID RVVYDLDNPN661YTIPEEGDIL KFNSKFESGN LRKVIQIRKN EYDLILNSDI NSNHYHQWFY FEVSGMRPGV721AYRFNIINCE KSNSQFNYGM QPLMYSVQEA LNARPWWIRM GTDICYYKNH FSRSSVAAGG781QKGKSYYTIT FTVNFPHKDD VCYFAYHYPY TYSTLQMHLQ KLESAHNPQQ IYFRKDVLCE841TLSGNSCPLV TITAMPESNY YEHICHFRNR PYVFLSARVH PGETNASWVM KGTLEYLMSN901NPTAQSLRES YIFKIVPMLN PDGVINGNHR CSLSGEDLNR QWQSPSPDLH PTIYHAKGLL961QYLAAVKRLP LVYCDYHGHS RKKNVFMYGC SIKETVWHTN DNATSCDVVE DTGYRTLPKI1021LSHIAPAFCM SSCSFVVEKS KESTARVVVW REIGVQRSYT MESTLCGCDQ GKYKGLQIGT1081RELEEMGAKF CVGLLRLKRL TSPLEYNLPS SLLDFENDLI ESSCKVTSPT TYVLDEDEPR1141FLEEVDYSAE SNDELDIELA ENVGDYEPSA QEEVLSDSEL SRTYLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1186 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.