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NLRX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLRX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101895-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRX1 (NM_024618), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR11.3 | Caterpiller protein 11.3 | DLNB26 | NLR family member X1 | NLR family | X1 | NOC4L | NOD9 | NOD5 | NLRX1 | NOD-like receptor X1 | NOD26
Origin Species
Human
NCBI GenBank
NM_024618

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRWGHHLPRA SWGSGFRRAL QRPDDRIPFL IHWSWPLQGE RPFGPPRAFI RHHGSSVDSA
61PPPGRHGRLF PSASATEAIQ RHRRNLAEWF SRLPREERQF GPTFALDTVH VDPVIRESTP
121DELLRPPAEL ALEHQPPQAG LPPLALSQLF NPDACGRRVQ TVVLYGTVGT GKSTLVRKMV
181LDWCYGRLPA FELLIPFSCE DLSSLGPAPA SLCQLVAQRY TPLKEVLPLM AAAGSHLLFV
241LHGLEHLNLD FRLAGTGLCS DPEEPQEPAA IIVNLLRKYM LPQASILVTT RPSAIGRIPS
301KYVGRYGEIC GFSDTNLQKL YFQLRLNQPY CGYAVGGSGV SATPAQRDHL VQMLSRNLEG
361HHQIAAACFL PSYCWLVCAT LHFLHAPTPA GQTLTSIYTS FLRLNFSGET LDSTDPSNLS
421LMAYAARTMG KLAYEGVSSR KTYFSEEDVC GCLEAGIRTE EEFQLLHIFR RDALRFFLAP
481CVEPGRAGTF VFTVPAMQEY LAALYIVLGL RKTTLQKVGK EVAELVGRVG EDVSLVLGIM
541AKLLPLRALP LLFNLIKVVP RVFGRMVGKS REAVAQAMVL EMFREEDYYN DDVLDQMGAS
601ILGVEGPRRH PDEPPEDEVF ELFPMFMGGL LSAHNRAVLA QLGCPIKNLD ALENAQAIKK
661KLGKLGRQVL PPSELLDHLF FHYEFQNQRF SAEVLSSLRQ LNLAGVRMTP VKCTVVAAVL
721GSGRHALDEV NLASCQLDPA GLRTLLPVFL RARKLGLQLN SLGPEACKDL RDLLLHDQCQ
781ITTLRLSNNP LTAAGVAVLM EGLAGNTSVT HLSLLHTGLG DEGLELLAAQ LDRNRQLQEL
841NVAYNGAGDT AALALARAAR EHPSLELLHL YFNELSSEGR QVLRDLGGAA EGGARVVVSL
901TEGTAVSEYW SVILSEVQRN LNSWDRARVQ RHLELLLRDL EDSRGATLNP WRKAQLLRVE
961GEVRALLEQL GSSGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

975 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.