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NLRP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLRP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NLRP9 antibodies →
$725.00each
Catalog Number: LS-G104850-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRP9 (NM_176820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NALP9 | NLRP9 | PAN12 | CLR19.1 | NOD6
Origin Species
Human
SwissProt
Q7RTR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAESFFSDFG LLWYLKELRK EEFWKFKELL KQPLEKFELK PIPWAELKKA SKEDVAKLLD
61KHYPGKQAWE VTLNLFLQIN RKDLWTKAQE EMRNKLNPYR KHMKETFQLI WEKETCLHVP
121EHFYKETMKN EYKELNDAYT AAARRHTVVL EGPDGIGKTT LLRKVMLDWA EGNLWKDRFT
181FVFFLNVCEM NGIAETSLLE LLSRDWPESS EKIEDIFSQP ERILFIMDGF EQLKFNLQLK
241ADLSDDWRQR QPMPIILSSL LQKKMLPESS LLIALGKLAM QKHYFMLRHP KLIKLLGFSE
301SEKKSYFSYF FGEKSKALKV FNFVRDNGPL FILCHNPFTC WLVCTCVKQR LERGEDLEIN
361SQNTTYLYAS FLTTVFKAGS QSFPPKVNRA RLKSLCALAA EGIWTYTFVF SHGDLRRNGL
421SESEGVMWVG MRLLQRRGDC FAFMHLCIQE FCAAMFYLLK RPKDDPNPAI GSITQLVRAS
481VVQPQTLLTQ VGIFMFGIST EEIVSMLETS FGFPLSKDLK QEITQCLESL SQCEADREAI
541AFQELFIGLF ETQEKEFVTK VMNFFEEVFI YIGNIEHLVI ASFCLKHCQH LTTLRMCVEN
601IFPDDSGCIS DYNEKLVYWR ELCSMFITNK NFQILDMENT SLDDPSLAIL CKALAQPVCK
661LRKLIFTSVY FGHDSELFKA VLHNPHLKLL SLYGTSLSQS DIRHLCETLK HPMCKIEELI
721LGKCDISSEV CEDIASVLAC NSKLKHLSLV ENPLRDEGMT LLCEALKHSH CALERLMLMY
781CCLTSVSCDS ISEVLLCSKS LSLLDLGSNA LEDNGVASLC AALKHPGCSI RELWLMGCFL
841TSDSCKDIAA VLICNGKLKT LKLGHNEIGD TGVRQLCAAL QHPHCKLECL GLQTCPITRA
901CCDDIAAALI ACKTLRSLNL DWIALDADAV VVLCEALSHP DCALQMLGLH KSGFDEETQK
961ILMSVEEKIP HLTISHGPWI DEEYKIRGVL L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

991 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.