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NLRP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLRP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NLRP8 antibodies →
$725.00each
Catalog Number: LS-G104872-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRP8 (NM_176811), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR19.2 | NALP8 | NOD16 | PAN4 | NLRP8
Origin Species
Human
SwissProt
Q86W28

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDVNPPSDT PIPFSSSSTH SSHIPPWTFS CYPGSPCENG VMLYMRNVSH EELQRFKQLL
61LTELSTGTMP ITWDQVETAS WAEVVHLLIE RFPGRRAWDV TSNIFAIMNC DKMCVVVRRE
121INAILPTLEP EDLNVGETQV NLEEGESGKI RRYKSNVMEK FFPIWDITTW PGNQRDFFYQ
181GVHRHEEYLP CLLLPKRPQG RQPKTVAIQG APGIGKTILA KKVMFEWARN KFYAHKRWCA
241FYFHCQEVNQ TTDQSFSELI EQKWPGSQDL VSKIMSKPDQ LLLLLDGFEE LTSTLIDRLE
301DLSEDWRQKL PGSVLLSSLL SKTMLPEATL LIMIRFTSWQ TCKPLLKCPS LVTLPGFNTM
361EKIKYFQMYF GHTEEGDQVL SFAMENTILF SMCRVPVVCW MVCSGLKQQM ERGNNLTQSC
421PNATSVFVRY ISSLFPTRAE NFSRKIHQAQ LEGLCHLAAD SMWHRKWVLG KEDLEEAKLD
481QTGVTAFLGM SILRRIAGEE DHYVFTLVTF QEFFAALFYV LCFPQRLKNF HVLSHVNIQR
541LIASPRGSKS YLSHMGLFLF GFLNEACASA VEQSFQCKVS FGNKRKLLKV IPLLHKCDPP
601SPGSGVPQLF YCLHEIREEA FVSQALNDYH KVVLRIGNNK EVQVSAFCLK RCQYLHEVEL
661TVTLNFMNVW KLSSSSHPGS EAPESNGLHR WWQDLCSVFA TNDKLEVLTM TNSVLGPPFL
721KALAAALRHP QCKLQKLLLR RVNSTMLNQD LIGVLTGNQH LRYLEIQHVE VESKAVKLLC
781RVLRSPRCRL QCLRLEDCLA TPRIWTDLGN NLQGNGHLKT LILRKNSLEN CGAYYLSVAQ
841LERLSIENCN LTQLTCESLA SCLRQSKMLT HLSLAENALK DEGAKHIWNA LPHLRCPLQR
901LVLRKCDLTF NCCQDMISAL CKNKTLKSLD LSFNSLKDDG VILLCEALKN PDCTLQILEL
961ENCLFTSICC QAMASMLRKN QHLRHLDLSK NAIGVYGILT LCEAFSSQKK REEVIFCIPA
1021WTRITSFSPT PHPPDFTGKS DCLSQINP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1048 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.