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NLRP11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLRP11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NLRP11 antibodies →
$504.00each
Catalog Number: LS-G106858-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRP11 (NM_145007), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR19.6 | NALP11 | NLRP11 | PYPAF6 | PYPAF7 | NOD17 | PAN10
Origin Species
Human
SwissProt
P59045

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAESDSTDFD LLWYLENLSD KEFQSFKKYL ARKILDFKLP QFPLIQMTKE ELANVLPISY
61EGQYIWNMLF SIFSMMRKED LCRKIIGRRN RNQEACKAVM RRKFMLQWES HTFGKFHYKF
121FRDVSSDVFY ILQLAYDSTS YYSANNLNVF LMGERASGKT IVINLAVLRW IKGEMWQNMI
181SYVVHLTSHE INQMTNSSLA ELIAKDWPDG QAPIADILSD PKKLLFILED LDNIRFELNV
241NESALCSNST QKVPIPVLLV SLLKRKMAPG CWFLISSRPT RGNNVKTFLK EVDCCTTLQL
301SNGKREIYFN SFFKDRQRAS AALQLVHEDE ILVGLCRVAI LCWITCTVLK RQMDKGRDFQ
361LCCQTPTDLH AHFLADALTS EAGLTANQYH LGLLKRLCLL AAGGLFLSTL NFSGEDLRCV
421GFTEADVSVL QAANILLPSN THKDRYKFIH LNVQEFCTAI AFLMAVPNYL IPSGSREYKE
481KREQYSDFNQ VFTFIFGLLN ANRRKILETS FGYQLPMVDS FKWYSVGYMK HLDRDPEKLT
541HHMPLFYCLY ENREEEFVKT IVDALMEVTV YLQSDKDMMV SLYCLDYCCH LRTLKLSVQR
601IFQNKEPLIR PTASQMKSLV YWREICSLFY TMESLRELHI FDNDLNGISE RILSKALEHS
661SCKLRTLKLS YVSTASGFED LLKALARNRS LTYLSINCTS ISLNMFSLLH DILHEPTCQI
721SHLSLMKCDL RASECEEIAS LLISGGSLRK LTLSSNPLRS DGMNILCDAL LHPNCTLISL
781VLVFCCLTEN CCSALGRVLL FSPTLRQLDL CVNRLKNYGV LHVTFPLLFP TCQLEELHLS
841GCFFSSDICQ YIAIVIATNE KLRSLEIGSN KIEDAGMQLL CGGLRHPNCM LVNIGLEECM
901LTSACCRSLA SVLTTNKTLE RLNLLQNHLG NDGVAKLLES LISPDCVLKV VGLPLTGLNT
961QTQQLLMTVK ERKPSLIFLS ETWSLKEGRE IGVTPASQPG SIIPNSNLDY MFFKFPRMSA
1021AMRTSNTASR QPL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1033 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.