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NLK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101405-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLK (NM_016231), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LAK1 | Nemo-like kinase | Nmo kinase | NLK | Nemo like kinase | Protein LAK1
Origin Species
Human
NCBI GenBank
NM_016231
SwissProt
Q9UBE8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.08 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAYNGGTSA AATGHHHHHH HHLPHLPPPH LHHHHHPQHH LHPGSAAAVH PVQQHTSSAA
61AAAAAAAAAA AMLNPGQQQP YFPSPAPGQA PGPAAAAPAQ VQAAAAATVK AHHHQHSHHP
121QQQLDIEPDR PIGYGAFGVV WSVTDPRDGK RVALKKMPNV FQNLVSCKRV FRELKMLCFF
181KHDNVLSALD ILQPPHIDYF EEIYVVTELM QSDLHKIIVS PQPLSSDHVK VFLYQILRGL
241KYLHSAGILH RDIKPGNLLV NSNCVLKICD FGLARVEELD ESRHMTQEVV TQYYRAPEIL
301MGSRHYSNAI DIWSVGCIFA ELLGRRILFQ AQSPIQQLDL ITDLLGTPSL EAMRTACEGA
361KAHILRGPHK QPSLPVLYTL SSQATHEAVH LLCRMLVFDP SKRISAKDAL AHPYLDEGRL
421RYHTCMCKCC FSTSTGRVYT SDFEPVTNPK FDDTFEKNLS SVRQVKEIIH QFILEQQKGN
481RVPLCINPQS AAFKSFISST VAQPSEMPPS PLVWE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

515 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.