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NLGN4Y Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLGN4Y Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113613-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neuroligin 4(NLGN4Y) (NM_014893), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neuroligin 4 | Y linked | Y-linked | Neuroligin Y | KIAA0951 | Neuroligin-4 | NLGN4Y
Origin Species
Human
SwissProt
Q8NFZ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRPQGLLWL PLLFTSVCVM LNSNVLLWIT ALAIKFTLID SQAQYPVVNT NYGKIQGLRT
61PLPSEILGPV EQYLGVPYAS PPTGERRFQP PESPSSWTGI RNATQFSAVC PQHLDERFLL
121HDMLPIWFTT SLDTLMTYVQ DQNEDCLYLN IYVPMEDDIH EQNSKKPVMV YIHGGSYMEG
181TGNMIDGSIL ASYGNVIVIT INYRLGILGF LSTGDQAAKG NYGLLDQIQA LRWIEENVGA
241FGGDPKRVTI FGSGAGASCV SLLTLSHYSE GLFQKAIIQS GTALSSWAVN YQPAKYTRIL
301ADKVGCNMLD TTDMVECLKN KNYKELIQQT ITPATYHIAF GPVIDGDVIP DDPQILMEQG
361EFLNYDIMLG VNQGEGLKFV DGIVDNEDGV TPNDFDFSVS NFVDNLYGYP EGKDTLRETI
421KFMYTDWADK ENPETRRKTL VALFTDHQWV APAVATADLH AQYGSPTYFY AFYHHCQSEM
481KPSWADSAHG DEVPYVFGIP MIGPTELFSC NFSKNDVMLS AVVMTYWTNF AKTGDPNQPV
541PQDTKFIHTK PNRFEEVAWS KYNPKDQLYL HIGLKPRVRD HYRATKVAFW LELVPHLHNL
601NEIFQYVSTT TKVPPPDMTS FPYGTRRSPA KIWPTTKRPA ITPANNPKHS KDPHKTGPED
661TTVLIETKRD YSTELSVTIA VGASLLFLNI LAFAALYYKK DKRRHETHRH PSPQRNTTND
721ITHIQNEEIM SLQMKQLEHD HECESLQAHD TLRLTCPPDY TLTLRRSPDD IPFMTPNTIT
781MIPNTLMGMQ PLHTFKTFSG GQNSTNLPHG HSTTRV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

816 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.