Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NLGN3 / Neuroligin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101606-100
Catalog Number: LS-G101606
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neuroligin 3(NLGN3) (NM_018977), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASPGX1 | AUTSX1 | Gliotactin homolog | KIAA1480 | NLGN3 | HNL3 | Neuroligin 3 | Neuroligin-3 | NL3
- Origin Species
- Human
- NCBI GenBank
- NM_018977
- SwissProt
- Q9NZ94
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLRLGPPSL SLSPKPTVGR SLCLTLWFLS LALRASTQAP APTVNTHFGK LRGARVPLPS61EILGPVDQYL GVPYAAPPIG EKRFLPPEPP PYWSGIRNAT HFPPVCPQNI HTAVPEVMLP121VWFTANLDIV ATYIQEPNED CLYLNVYVPT EDGSGAKKQG EDLADNDGDE DEDIRDSGAK181PVMVYIHGGS YMEGTGNMID GSILASYGNV IVITLNYRVG VLGFLSTGDQ AAKGNYGLLD241QIQALRWVSE NIAFFGGDPR RITVFGSGIG ASCVSLLTLS HHSEGLFQRA IIQSGSALSS301WAVNYQPVKY TSLLADKVGC NVLDTVDMVD CLRQKSAKEL VEQDIQPARY HVAFGPVIDG361DVIPDDPEIL MEQGEFLNYD IMLGVNQGEG LKFVEGVVDP EDGVSGTDFD YSVSNFVDNL421YGYPEGKDTL RETIKFMYTD WADRDNPETR RKTLVALFTD HQWVEPSVVT ADLHARYGSP481TYFYAFYHHC QSLMKPAWSD AAHGDEVPYV FGVPMVGPTD LFPCNFSKND VMLSAVVMTY541WTNFAKTGDP NKPVPQDTKF IHTKANRFEE VAWSKYNPRD QLYLHIGLKP RVRDHYRATK601VAFWKHLVPH LYNLHDMFHY TSTTTKVPPP DTTHSSHITR RPNGKTWSTK RPAISPAYSN661ENAQGSWNGD QDAGPLLVEN PRDYSTELSV TIAVGASILF LNVLAFAALY YRKDKRRQEP721LRQPSPQRGA WAPELGAAPE EELAALQLGP THHECEASPP HDTLRLTALP DYTLTLRRSP781DDIPLMTPNT ITMIPNSLVG LQTLHPYNTF AAGFNSTGLP HSHSTTRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
828 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.