Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NLGN1 / Neuroligin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101277-100
Catalog Number: LS-G101277
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neuroligin 1(NLGN1) (NM_014932), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1070 | Neuroligin-1 | NLGN1 | Neuroligin I | Neuroligin 1 | NL1
- Origin Species
- Human
- NCBI GenBank
- NM_014932
- SwissProt
- Q8N2Q7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALPRCTWPN YVWRAVMACL VHRGLGAPLT LCMLGCLLQA GHVLSQKLDD VDPLVATNFG61KIRGIKKELN NEILGPVIQF LGVPYAAPPT GERRFQPPEP PSPWSDIRNA TQFAPVCPQN121IIDGRLPEVM LPVWFTNNLD VVSSYVQDQS EDCLYLNIYV PTEDDIRDSG GPKPVMVYIH181GGSYMEGTGN LYDGSVLASY GNVIVITVNY RLGVLGFLST GDQAAKGNYG LLDLIQALRW241TSENIGFFGG DPLRITVFGS GAGGSCVNLL TLSHYSEGNR WSNSTKGLFQ RAIAQSGTAL301SSWAVSFQPA KYARMLATKV GCNVSDTVEL VECLQKKPYK ELVDQDIQPA RYHIAFGPVI361DGDVIPDDPQ ILMEQGEFLN YDIMLGVNQG EGLKFVENIV DSDDGISASD FDFAVSNFVD421NLYGYPEGKD VLRETIKFMY TDWADRHNPE TRRKTLLALF TDHQWVAPAV ATADLHSNFG481SPTYFYAFYH HCQTDQVPAW ADAAHGDEVP YVLGIPMIGP TELFPCNFSK NDVMLSAVVM541TYWTNFAKTG DPNQPVPQDT KFIHTKPNRF EEVAWTRYSQ KDQLYLHIGL KPRVKEHYRA601NKVNLWLELV PHLHNLNDIS QYTSTTTKVP STDITFRPTR KNSVPVTSAF PTAKQDDPKQ661QPSPFSVDQR DYSTELSVTI AVGASLLFLN ILAFAALYYK KDKRRHDVHR RCSPQRTTTN721DLTHAQEEEI MSLQMKHTDL DHECESIHPH EVVLRTACPP DYTLAMRRSP DDVPLMTPNT781ITMIPNTIPG IQPLHTFNTF TGGQNNTLPH PHPHPHSHST TRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
823 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.