Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NKRF / NRF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101482-100
Catalog Number: LS-G101482
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NKRF (NM_017544), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NFkB-repressing factor | NKRF | ITBA4 | Protein ITBA4 | NF-kappaB repressing factor | NFKB repressing factor | Transcription factor NRF | NF-kappa-B-repressing factor | NRF
- Origin Species
- Human
- NCBI GenBank
- NM_017544
- SwissProt
- O15226
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK FHARPRFEPV61HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY TQDSFSIQDG NSQYCDSSGF121ILTKDQPVTA NMYFDSGNPA PSTTSQQANS QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT181IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY241ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI301GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI GILNNSASFN361KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKILQ KTQPTYPSVK421SSQCHTGSSP RGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC481KVILESEVIA EAVGVKKTVK YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS541AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK601IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV661GRKRRKEDLL DQLKQEGQVG HYELVMPQANTRTRPLEQKLISEEDLAANDILDYKDDDDKV
690 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.