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NKPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NKPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NKPD1 antibodies →
$504.00each
Catalog Number: LS-G103678-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NKPD1 (NM_198478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NKPD1
Origin Species
Human
NCBI GenBank
NM_198478
SwissProt
Q17RQ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQQEAAQRES EELQHVQWRP RAVSGWGVPQ LLWYLVFLQP IITEVHLRRR NVQFLFIRFS
61AWQYAGTDKL WAGLVTTLCE GIRRHYGALP FSVYSVLGNK PATRQDCCQS EWHCRRRVCL
121GLLALLAALG LGVGLLYLSL GGHALGHGSP SGSLLKVFGG AATTLSGSGL LMAVYSVGKH
181LFVSQRKKIE RLVSREKFGS QLGFMCEVKK EVELLTDFLC FLEIYQRRRL RVVLEVTGLD
241TCYPERVVGV LNAINTLLSD SHAPFIFILV VDPSILAACL ESAGNMKGTA DNGYLFLNRT
301VTLPFSVPIM GRRTKLQFLH DAVQSRDDLL YREMTRKPWL PGDAGGESAQ LLAVQAQAGT
361ERGQGRIDDE AARRIQEALF CLHDERDCLY EYVPDNVVSM RRIVNTVPIT VRLLQQQQQQ
421GDFGGPTPRQ AVAWVVLANQ WPCRLSWALQ CLEDRQQTGG APEGRARLWD VFRDNSRELH
481TMTKALQNVL DLDGDPELFE RFLGADFPFT VAEAQSLLRC TVNLDHSIRR RMGLIRAVSA
541LKPPSPPKSP TRDTPHAAHR ANSASRAPPS GRASGQAGEG HHTGDLAHRG KLWPVACALF
601RPGQSSPGGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.