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NKAP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NKAP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101880-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NKAP (NM_024528), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NF-kappaB activating protein | NFKB activating protein | NF-kappa-B-activating protein | NKAP
Origin Species
Human
NCBI GenBank
NM_024528
SwissProt
Q8N5F7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS GDRNGLTHQL
61GGLSQGSRNQ SYRSRSRSRS RERPSAPRGI PFASASSSVY YGSYSRPYGS DKPWPSLLDK
121EREESLRQKR LSERERIGEL GAPEVWGLSP KNPEPDSDEH TPVEDEEPKK STTSASTSEE
181EKKKKSSRSK ERSKKRRKKK SSKRKHKKYS EDSDSDSDSE TDSSDEDNKR RAKKAKKKEK
241KKKHRSKKYK KKRSKKSRKE SSDSSSKESQ EEFLENPWKY RTKAEEPSDL IGPEAPKTLT
301SQDDKPLNYG HALLPGEGAA MAEYVKAGKR IPRRGEIGLT SEEIASFECS GYVMSGSRHR
361RMEAVRLRKE NQIYSADEKR ALASFNQEER RKRENKILAS FREMVYRKTK GKDDK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

415 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.