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NISCH Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NISCH Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114799-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NISCH (NM_007184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIRAS | I-1 | I-1 receptor candidate protein | Imidazoline receptor candidate | Imidazoline receptor 1 | IRAS | KIAA0975 | IR1 | NISCH | Nischarin | I1R | I1R candidate protein | Imidazoline-1 receptor
Origin Species
Human
NCBI GenBank
NM_007184
SwissProt
Q9Y2I1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
166.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATARTFGPE REAEPAKEAR VVGSELVDTY TVYIIQVTDG SHEWTVKHRY SDFHDLHEKL
61VAERKIDKNL LPPKKIIGKN SRSLVEKREK DLEVYLQKLL AAFPGVTPRV LAHFLHFHFY
121EINGITAALA EELFEKGEQL LGAGEVFAIG PLQLYAVTEQ LQQGKPTCAS GDAKTDLGHI
181LDFTCRLKYL KVSGTEGPFG TSNIQEQLLP FDLSIFKSLH QVEISHCDAK HIRGLVASKP
241TLATLSVRFS ATSMKEVLVP EASEFDEWEP EGTTLEGPVT AVIPTWQALT TLDLSHNSIS
301EIDESVKLIP KIEFLDLSHN GLLVVDNLQH LYNLVHLDLS YNKLSSLEGL HTKLGNIKTL
361NLAGNLLESL SGLHKLYSLV NLDLRDNRIE QMEEVRSIGS LPCLEHVSLL NNPLSIIPDY
421RTKVLAQFGE RASEVCLDDT VTTEKELDTV EVLKAIQKAK EVKSKLSNPE KKGGEDSRLS
481AAPCIRPSSS PPTVAPASAS LPQPILSNQG IMFVQEEALA SSLSSTDSLT PEHQPIAQGC
541SDSLESIPAG QAASDDLRDV PGAVGGASPE HAEPEVQVVP GSGQIIFLPF TCIGYTATNQ
601DFIQRLSTLI RQAIERQLPA WIEAANQREE GQGEQGEEED EEEEEEEDVA ENRYFEMGPP
661DVEEEEGGGQ GEEEEEEEED EEAEEERLAL EWALGADEDF LLEHIRILKV LWCFLIHVQG
721SIRQFAACLV LTDFGIAVFE IPHQESRGSS QHILSSLRFV FCFPHGDLTE FGFLMPELCL
781VLKVRHSENT LFIISDAANL HEFHADLRSC FAPQHMAMLC SPILYGSHTS LQEFLRQLLT
841FYKVAGGCQE RSQGCFPVYL VYSDKRMVQT AAGDYSGNIE WASCTLCSAV RRSCCAPSEA
901VKSAAIPYWL LLTPQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST VLLDPTRSCT
961QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ LRASLQDLKT VVIAKTPGTG
1021GSPQGSFADG QPAERRASND QRPQEVPAEA LAPAPAEVPA PAPAAASASG PAKTPAPAEA
1081STSALVPEET PVEAPAPPPA EAPAQYPSEH LIQATSEENQ IPSHLPACPS LRHVASLRGS
1141AIIELFHSSI AEVENEELRH LMWSSVVFYQ TPGLEVTACV LLSTKAVYFV LHDGLRRYFS
1201EPLQDFWHQK NTDYNNSPFH ISQCFVLKLS DLQSVNVGLF DQHFRLTGST PMQVVTCLTR
1261DSYLTHCFLQ HLMVVLSSLE RTPSPEPVDK DFYSEFGNKT TGKMENYELI HSSRVKFTYP
1321SEEEIGDLTF TVAQKMAEPE KAPALSILLY VQAFQVGMPP PGCCRGPLRP KTLLLTSSEI
1381FLLDEDCVHY PLPEFAKEPP QRDRYRLDDG RRVRDLDRVL MGYQTYPQAL TLVFDDVQGH
1441DLMGSVTLDH FGEVPGGPAR ASQGREVQWQ VFVPSAESRE KLISLLARQW EALCGRELPV
1501ELTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1504 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.