Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NIPAL3 / NPAL3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111137-100
Catalog Number: LS-G111137
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NIPAL3 (NM_020448), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ462O23.2 | RP3-462O23.3 | NIPA-like domain containing 3 | NIPA-like protein 3 | NIPAL3 | NPAL3
- Origin Species
- Human
- NCBI GenBank
- NM_020448
- SwissProt
- Q6P499
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGSHSAALK LQQLPPTSSS SAVSEASFSY KENLIGALLA IFGHLVVSIA LNLQKYCHIR61LAGSKDPRAY FKTKTWWLGL FLMLLGELGV FASYAFAPLS LIVPLSAVSV IASAIIGIIF121IKEKWKPKDF LRRYVLSFVG CGLAVVGTYL LVTFAPNSHE KMTGENVTRH LVSWPFLLYM181LVEIILFCLL LYFYKEKNAN NIVVILLLVA LLGSMTVVTV KAVAGMLVLS IQGNLQLDYP241IFYVMFVCMV ATAVYQAAFL SQASQMYDSS LIASVGYILS TTIAITAGAI FYLDFIGEDV301LHICMFALGC LIAFLGVFLI TRNRKKPIPF EPYISMDAMP GMQNMHDKGM TVQPELKASF361SYGALENNDN ISEIYAPATL PVMQEEHGSR SASGVPYRVL EHTKKESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
406 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.