Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NIP45 / NFATC2IP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108637-100
Catalog Number: LS-G108637
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFATC2IP (NM_032815), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ESC2 | NFATC2IP | NIP45 | NFATC2-interacting protein | RAD60
- Origin Species
- Human
- NCBI GenBank
- NM_032815
- SwissProt
- Q8NCF5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEPVGKRGR WSGGSGAGRG GRGGWGGRGR RPRAQRSPSR GTLDVVSVDL VTDSDEEILE61VATARGAADE VEVEPPEPPG PVASRDNSNS DSEGEDRRPA GPPREPVRRR RRLVLDPGEA121PLVPVYSGKV KSSLRLIPDD LSLLKLYPPG DEEEAELADS SGLYHEGSPS PGSPWKTKLR181TKDKEEKKKT EFLDLDNSPL SPPSPRTKSR THTRALKKLS EVNKRLQDLR SCLSPKPPQG241QEQQGQEDEV VLVEGPTLPE TPRLFPLKIR CRADLVRLPL RMSEPLQSVV DHMATHLGVS301PSRILLLFGE TELSPTATPR TLKLGVADII DCVVLTSSPE ATETSQQLQL RVQGKEKHQT361LEVSLSRDSP LKTLMSHYEE AMGLSGRKLS FFFDGTKLSG RELPADLGME SGDLIEVWGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
419 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.