Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NIM1K / NIM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105805-100
Catalog Number: LS-G105805
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NIM1K(NIM1K) (NM_153361), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MGC42105 | NIM1
- Origin Species
- Human
- NCBI GenBank
- NM_153361
- SwissProt
- Q8IY84
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAVYMNGGG LVNPHYARWD RRDSVESGCQ TESSKEGEEG QPRQLTPFEK LTQDMSQDEK61VVREITLGKR IGFYRIRGEI GSGNFSQVKL GIHSLTKEKV AIKILDKTKL DQKTQRLLSR121EISSMEKLHH PNIIRLYEVV ETLSKLHLVM EYAGGGELFG KISTEGKLSE PESKLIFSQI181VSAVKHMHEN QIIHRDLKAE NVFYTSNTCV KVGDFGFSTV SKKGEMLNTF CGSPPYAAPE241LFRDEHYIGI YVDIWALGVL LYFMVTGTMP FRAETVAKLK KSILEGTYSV PPHVSEPCHR301LIRGVLQQIP TERYGIDCIM NDEWMQGVPY PTPLEPFQLD PKHLSETSTL KEEENEVKST361LEHLGITEEH IRNNQGRDAR SSITGVYRII LHRVQRKKAL ESVPVMMLPD PKERDLKKGS421RVYRGIRHTS KFCSILTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.