Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NID2 / Nidogen-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114675-100
Catalog Number: LS-G114675
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nidogen 2(NID2) (NM_007361), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NID2 | Nidogen 2 (osteonidogen) | NID-2 | Osteonidogen | Nidogen-2
- Origin Species
- Human
- NCBI GenBank
- NM_007361
- SwissProt
- Q14112
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 151.29 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGDRVAGRP VLSSLPVLLL LQLLMLRAAA LHPDELFPHG ESWGDQLLQE GDDESSAVVK61LANPLHFYEA RFSNLYVGTN GIISTQDFPR ETQYVDYDFP TDFPAIAPFL ADIDTSHGRG121RVLYREDTSP AVLGLAARYV RAGFPRSARF TPTHAFLATW EQVGAYEEVK RGALPSGELN181TFQAVLASDG SDSYALFLYP ANGLQFLGTR PKESYNVQLQ LPARVGFCRG EADDLKSEGP241YFSLTSTEQS VKNLYQLSNL GIPGVWAFHI GSTSPLDNVR PAAVGDLSAA HSSVPLGRSF301SHATALESDY NEDNLDYYDV NEEEAEYLPG EPEEALNGHS SIDVSFQSKV DTKPLEESST361LDPHTKEGTS LGEVGGPDLK GQVEPWDERE TRSPAPPEVD RDSLAPSWET PPPYPENGSI421QPYPDGGPVP SEMDVPPAHP EEEIVLRSYP ASGHTTPLSR GTYEVGLEDN IGSNTEVFTY481NAANKETCEH NHRQCSRHAF CTDYATGFCC HCQSKFYGNG KHCLPEGAPH RVNGKVSGHL541HVGHTPVHFT DVDLHAYIVG NDGRAYTAIS HIPQPAAQAL LPLTPIGGLF GWLFALEKPG601SENGFSLAGA AFTHDMEVTF YPGEETVRIT QTAEGLDPEN YLSIKTNIQG QVPYVPANFT661AHISPYKELY HYSDSTVTST SSRDYSLTFG AINQTWSYRI HQNITYQVCR HAPRHPSFPT721TQQLNVDRVF ALYNDEERVL RFAVTNQIGP VKEDSDPTPG NPCYDGSHMC DTTARCHPGT781GVDYTCECAS GYQGDGRNCV DENECATGFH RCGPNSVCIN LPGSYRCECR SGYEFADDRH841TCILITPPAN PCEDGSHTCA PAGQARCVHH GGSTFSCACL PGYAGDGHQC TDVDECSENR901CHPAATCYNT PGSFSCRCQP GYYGDGFQCI PDSTSSLTPC EQQQRHAQAQ YAYPGARFHI961PQCDEQGNFL PLQCHGSTGF CWCVDPDGHE VPGTQTPPGS TPPHCGPSPE PTQRPPTICE1021RWRENLLEHY GGTPRDDQYV PQCDDLGHFI PLQCHGKSDF CWCVDKDGRE VQGTRSQPGT1081TPACIPTVAP PMVRPTPRPD VTPPSVGTFL LYTQGQQIGY LPLNGTRLQK DAAKTLLSLH1141GSIIVGIDYD CRERMVYWTD VAGRTISRAG LELGAEPETI VNSGLISPEG LAIDHIRRTM1201YWTDSVLDKI ESALLDGSER KVLFYTDLVN PRAIAVDPIR GNLYWTDWNR EAPKIETSSL1261DGENRRILIN TDIGLPNGLT FDPFSKLLCW ADAGTKKLEC TLPDGTGRRV IQNNLKYPFS1321IVSYADHFYH TDWRRDGVVS VNKHSGQFTD EYLPEQRSHL YGITAVYPYC PTGRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1375 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.