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Niban-Like Protein 1 (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Niban-Like Protein 1 (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM129B antibodies →
$725.00each
Catalog Number: LS-G110216-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM129B (NM_022833), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA356B19.6 | C9orf88 | DKFZP434H0820 | FAM129B | MEG-3 | MINERVA | Protein FAM129B | Melanoma invasion by ERK | Niban-like protein 1 | OC58
Origin Species
Human
NCBI GenBank
NM_022833
SwissProt
Q96TA1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDVLSTHLD DARRQHIAEK TGKILTEFLQ FYEDQYGVAL FNSMRHEIEG TGLPQAQLLW
61RKVPLDERIV FSGNLFQHQE DSKKWRNRFS LVPHNYGLVL YENKAAYERQ VPPRAVINSA
121GYKILTSVDQ YLELIGNSLP GTTAKSGSAP ILKCPTQFPL ILWHPYARHY YFCMMTEAEQ
181DKWQAVLQDC IRHCNNGIPE DSKVEGPAFT DAIRMYRQSK ELYGTWEMLC GNEVQILSNL
241VMEELGPELK AELGPRLKGK PQERQRQWIQ ISDAVYHMVY EQAKARFEEV LSKVQQVQPA
301MQAVIRTDMD QIITSKEHLA SKIRAFILPK AEVCVRNHVQ PYIPSILEAL MVPTSQGFTE
361VRDVFFKEVT DMNLNVINEG GIDKLGEYME KLSRLAYHPL KMQSCYEKME SLRLDGLQQR
421FDVSSTSVFK QRAQIHMREQ MDNAVYTFET LLHQELGKGP TKEELCKSIQ RVLERVLKKY
481DYDSSSVRKR FFREALLQIS IPFLLKKLAP TCKSELPRFQ ELIFEDFARF ILVENTYEEV
541VLQTVMKDIL QAVKEAAVQR KHNLYRDSMV MHNSDPNLHL LAEGAPIDWG EEYSNSGGGG
601SPSPSTPESA TLSEKRRRAK QVVSVVQDEE VGLPFEASPE SPPPASPDGV TEIRGLLAQG
661LRPESPPPAG PLLNGAPAGE SPQPKAAPEA SSPPASPLQH LLPGKAVDLG PPKPSDQETG
721EQVSSPSSHP ALHTTTEDSA GVQTEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

746 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.