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NHSL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NHSL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NHSL2 antibodies →
$725.00each
Catalog Number: LS-G129165-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NHSL2 (NM_001013627), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001013627

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPFYRRTVVP QRLCPRNPPQ QLAELRDVSH LAALSLLRQL ADLCGHSLAL LEDLEGHLLA
61LGRRTDSLYR RTVRLRRRLP CRLLGPEEDE EELAAANSGR ENATATAHSR SSWRQPVNVF
121LSSGRPPSVE ELLREAQLNL QSLLQEEYEE QYSEARLVGQ TFRSSDEATK PTPNPRPQSA
181RRLEFILMPT KRQLSEDETT TQGVRAPEAS LSLSTTADKQ TAWNSLFPLP ILEEKRWPQL
241CSTQSDIVPI NISGQQFDKH ASLRHSLFNT ETAVNPKSTL RRRRTIIGFS NFSQRDQGHS
301NSPAGSVAHS TTSDIRPSHS VPEGVHGRVA VGQDARFPSL TSPVLRTPSS EPDEPHQARS
361GPNPPGMESM GMVYSVPSSC NGPTESTFST SWKGDAFTYM TPSATSQSNQ VNENGKNPSC
421GNSWVSLNKV PPLVPKEAAT LLVARDNPAG CSGSAGYPER LIQQRHMPER PSKIGLLTSG
481TSRLETGPGG ASRFRERSLS VPTDSGTTDV DYDEEQKANE ACALPFASTS SEGSNSADNI
541ASLSAQQEAQ HRRQRSKSIS LRKAKKKPSP PTRSVSLVKD EPGLLPEGGS ALPKDQRPKS
601LCLSLEHQGH HSSHPDAQGH PAIPNHKDPE STQFSHHWYL TDWKSGDTYQ SLSSSSTATG
661TTVIECTQVQ GSSESLASPS TSRATTPSQL SIEVEAREIS SPGRPPGLMS PSSGYSSQSE
721TPTPTVSMSL TLGHLPPPSS SVRVRPVVPE RKSSLPPTSP MEKFPKSRLS FDLPLTSSPN
781LDLSGMSISI RSKTKVSRHH SETNFGVKLA QKTNPNQPIM PMVTQSDLRS VRLRSVSKSE
841PEDDIESPEY AEEPRAEEVF TLPERKTKPP VAEKPPVARR PPSLVHKPPS VPEEYALTSP
901TLAMPPRSSI QHARPLPQDS YTVVRKPKPS SFPDGRSPGE STAPSSLVFT PFASSSDAFF
961SGTQQPPQGS VEDEGPKVRV LPERISLQSQ EEAEKKKGKI PPPVPKKPSV LYLPLTSPTA
1021QMEAYVAEPR LPLSPIITLE EDTKCPATGD DLQSLGQRVT STPQADSERE ASPLGSSVEP
1081GTEEKSLISD KTAEWIAEDD DDVFVASRTT EDLFTVIHRS KRKLLGWKEP GEAFVGGRTS
1141SHSPIKNTAE SPISESTATA GSGSSANLDA GRNDDFKALL QKKGSKATPR SRPSAAELLK
1201TTNPLARRII AQFSKDYETT DNPST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1225 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.