LSBio
NHLRC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NHLRC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NHLRC2 antibodies →
$725.00each
Catalog Number: LS-G103667-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NHLRC2 (NM_198514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NHL repeat containing 2 | NHLRC2
Origin Species
Human
NCBI GenBank
NM_198514
SwissProt
Q8NBF2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPGGRGRS LSGLLPAQTS LEYALLDAVT QQEKDSLVYQ YLQKVDGWEQ DLSVPEFPEG
61LEWLNTEEPI SVYKDLCGKI VVLDFFTYCC INCIHLLPDL HALEHTYSDK DGLLIIGVHS
121AKFPNEKVLD NIKSAVLRYN ITHPMVNDAD ASLWQELEVS CWPTLVILGP RGNMLFSLIG
181EGHKDKLFLY TSIALKYYKD RGQIRDNKIG IKLYKDSLPP SPLLFPGKVT VDQVTDRLVI
241ADTGHHRILV VWKNGQIQYS IGGPNPGRKD GIFSESTFNS PQGVAIMNNI IYVADTENHL
301IRKIDLEAEK VSTVAGIGIQ GTDKEGGAKG EQQPISSPWD VVFGTSGSEV QRGDILWIAM
361AGTHQIWALL LDSGKLPKKN ELTKGTCLRF AGSGNEENRN NAYPHKAGFA QPSGLSLASE
421DPWSCLFVAD SESSTVRTVS LKDGAVKHLV GGERDPMNLF AFGDVDGVGI NAKLQHPLGV
481TWDKKRNLLY VADSYNHKIK VVDPKTKNCT TLAGTGDTNN VTSSSFTEST FNEPGGLCIG
541ENGELLYVAD TNNHQIKVMD LETKMVSVLP IFRSENAVVD GPFLVEKQKT LPKLPKSAPS
601IRLSPVTACA GQTLQFKLRL DLPSGSKLTE GVSSCWFLTA EGNEWLLQGQ IAAGDIENIS
661SQPTISLQIP DDCLSLEAIV SVSVFLYYCS ADSSACMMKA ILFSQPLQIT DTQQGCIAPV
721ELRYVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

726 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.