LSBio
NGLY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NGLY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NGLY1 antibodies →
$504.00each
Catalog Number: LS-G126624-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NGLY1 (NM_001145293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Peptide:N-glycanase | N-glycanase 1 | NGLY1 | PNG1 | HPNGase | PNGase
Origin Species
Human
NCBI GenBank
NM_018297
SwissProt
Q96IV0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK YRSIRIGNTA
61FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAIERS SRLDGSNKSH
121KVKSSQQPAA STQLPTTPSS NPSGLNQHTR NRQGQSSDPP SASTVAADSA ILEVLQSNIQ
181HVLVYENPAL QEKALACIPV QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE
241FFHWVNNVLC SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
301LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTELQRTL SLKLTTLKEI GKTFLRISKR
361QKLIQVVDVT WRYSCKHEEV IARRTKVKEA LLRDTINGLN KQRQLFLSEN RRKELLQRII
421VELVEFISPK TPKPGELGGR ISGSVAWRVA RGEMGLQRKE TLFIPCENEK ISKQLHLCYN
481IVKDRYVRVS NNNQTISGWE NGVWKMESIF RKVETDWHMV YLARKEGSSF AYISWKFECG
541SVGLKVDSIS IRTSSQTFQT GTVEWKLRSD TAQVELTGDN SLHSYADFSG ATEVILEAEL
601SRGDGDVAWQ HTQLFRQSLN DHEENCLEII IKFSDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

636 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.