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NFRKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NFRKB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NFRKB antibodies →
$725.00each
Catalog Number: LS-G115489-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFRKB (NM_006165), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA-binding protein R kappa B | INO80G | NFRKB | DNA-binding protein R kappa-B | INO80 complex subunit G
Origin Species
Human
NCBI GenBank
NM_006165
SwissProt
Q6P4R8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHVFVQSTCG EETMDSLDHM LTDPLELGPC GDGHGTRIME DCLLGGTRVS LPEDLLEDPE
61IFFDVVSLST WQEVLSDSQR EHLQQFLPQF PEDSAEQQNE LILALFSGEN FRFGNPLHIA
121QKLFRDGHFN PEVVKYRQLC FKSQYKRYLN SQQQYFHRLL KQILASRSDL LEMARRSGPA
181LPFRQKRPSP SRTPEEREWR TQQRYLKVLR EVKEECGDTA LSSDEEATLD LQEQFSFEDL
241SSWLPSSPAR SPSPAVPLRV VPTLSTTDMK TADKVELGDS DLKIMLKKHH EKRKHQPDHP
301DLLTGDLTLN DIMTRVNAGR KGSLAALYDL AVLKKKVKEK EEKKKKKIKT IKSEAEDLAE
361PLSSTEGVAP LSQAPSPLAI PAIKEEPLED LKPCLGINEI SSSFFSLLLE ILLLESQASL
421PMLEERVLDW QSSPASSLNS WFSAAPNWAE LVLPALQYLA GESRAVPSSF SPFVEFKEKT
481QQWKLLGQSQ DNEKELAALF QLWLETKDQA FCKQENEDSS DATTPVPRVR TDYVVRPSTG
541EEKRVFQEQE RYRYSQPHKA FTFRMHGFES VVGPVKGVFD KETSLNKARE HSLLRSDRPA
601YVTILSLVRD AAARLPNGEG TRAEICELLK DSQFLAPDVT STQVNTVVSG ALDRLHYEKD
661PCVKYDIGRK LWIYLHRDRS EEEFERIHQA QAAAAKARKA LQQKPKPPSK VKSSSKESSI
721KVLSSGPSEQ SQMSLSDSSM PPTPVTPVTP TTPALPAIPI SPPPVSAVNK SGPSTVSEPA
781KSSSGVLLVS SPTMPHLGTM LSPASSQTAP SSQAAARVVS HSGSAGLSQV RVVAQPSLPA
841VPQQSGGPAQ TLPQMPAGPQ IRVPATATQT KVVPQTVMAT VPVKAQTTAA TVQRPGPGQT
901GLTVTSLPAT ASPVSKPATS SPGTSAPSAS TAAVIQNVTG QNIIKQVAIT GQLGVKPQTG
961NSIPLTATNF RIQGKDVLRL PPSSITTDAK GQTVLRITPD MMATLAKSQV TTVKLTQDLF
1021GTGGNTTGKG ISATLHVTSN PVHAADSPAK ASSASAPSST PTGTTVVKVT PDLKPTEASS
1081SAFRLMPALG VSVADQKGKS TVASSEAKPA ATIRIVQGLG VMPPKAGQTI TVATHAKQGA
1141SVASGSGTVH TSAVSLPSMN AAVSKTVAVA SGAASTPISI STGAPTVRQV PVSTTVVSTS
1201QAGKLPTRIT VPLSVISQPM KGKSVVTAPI IKGNLGANLS GLGRNIILTT MPAGTKLIAG
1261NKPVSFLTAQ QLQQLQQQGQ ATQVRIQTVP ASHLQQGTAS GSSKAVSTVV VTTAPSPKQA
1321PEQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1324 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.