Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFKBIA / IKB Alpha / IKBA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111114-100
Catalog Number: LS-G111114
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IKB alpha(NFKBIA) (NM_020529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IkB-alpha | IKBA | IkappaBalpha | MAD3 | NF-kappa-B inhibitor alpha | NFKBIA | NFKBI | I-kappa-B-alpha | IKB Alpha | MAD-3
- Origin Species
- Human
- NCBI GenBank
- NM_020529
- SwissProt
- P25963
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP61RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI121TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN181YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG241ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE301DELPYDDCVF GGQRLTLTRT RPLEQKLISE EDLAANDILD YKDDDDKV
348 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.