Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NFATC1 / NFAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102429-100
Catalog Number: LS-G102429
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFAT2(NFATC1) (NM_172390), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NFATc | NFAT2 | NFATC1 | NF-ATC | NF-ATC transcription factor | NF-ATc1
- Origin Species
- Human
- NCBI GenBank
- NM_006162
- SwissProt
- O95644
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSTSFPVPS KFPLGPAAAV FGRGETLGPA PRAGGTMKSA EEEHYGYASS NVSPALPLPT61AHSTLPAPCH NLQTSTPGII PPADHPSGYG AALDGGPAGY FLSSGHTRPD GAPALESPRI121EITSCLGLYH NNNQFFHDVE VEDVLPSSKR SPSTATLSLP SLEAYRDPSC LSPASSLSSR181SCNSEASSYE SNYSYPYASP QTSPWQSPCV SPKTTDPEEG FPRGLGACTL LGSPRHSPST241SPRASVTEES WLGARSSRPA SPCNKRKYSL NGRQPPYSPH HSPTPSPHGS PRVSVTDDSW301LGNTTQYTSS AIVAAINALT TDSSLDLGDG VPVKSRKTTL EQPPSVALKV EPVGEDLGSP361PPPADFAPED YSSFQHIRKG GFCDQYLAVP QHPYQWAKPK PLSPTSYMSP TLPALDWQLP421SHSGPYELRI EVQPKSHHRA HYETEGSRGA VKASAGGHPI VQLHGYLENE PLMLQLFIGT481ADDRLLRPHA FYQVHRITGK TVSTTSHEAI LSNTKVLEIP LLPENSMRAV IDCAGILKLR541NSDIELRKGE TDIGRKNTRV RLVFRVHVPQ PSGRTLSLQV ASNPIECSQR SAQELPLVEK601QSTDSYPVVG GKKMVLSGHN FLQDSKVIFV EKAPDGHHVW EMEAKTDRDL CKPNSLVVEI661PPFRNQRITS PVHVSFYVCN GKRKRSQYQR FTYLPANGNA IFLTVSREHE RVGCFFSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
716 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.