Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NF90 / ILF3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114559-100
Catalog Number: LS-G114559
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ILF3 (NM_012218), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CBTF | DRBF | DRBP76 | MMP4 | NF-AT-90 | NF90 | NFAR2 | Nuclear factor nf90 | ILF3 | M-phase phosphoprotein 4 | TCP110 | MPHOSPH4 | NF110b | NF90a | NF90b | NFAR-1 | TCP80 | NF110 | NFAR
- Origin Species
- Human
- NCBI GenBank
- NM_004516
- SwissProt
- Q12906
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRIFVNDDRH VMAKHSSVYP TQEELEAVQN MVSHTERALK AVSDWIDEQE KGSSEQAESD61NMDVPPEDDS KEGAGEQKTE HMTRTLRGVM RVGLVAKGLL LKGDLDLELV LLCKEKPTTA121LLDKVADNLA IQLAAVTEDK YEILQSVDDA AIVIKNTKEP PLSLTIHLTS PVVREEMEKV181LAGETLSVND PPDVLDRQKC LAALASLRHA KWFQARANGL KSCVIVIRVL RDLCTRVPTW241GPLRGWPLEL LCEKSIGTAN RPMGAGEALR RVLECLASGI VMPDGSGIYD PCEKEATDAI301GHLDRQQRED ITQSAQHALR LAAFGQLHKV LGMDPLPSKM PKKPKNENPV DYTVQIPPST361TYAITPMKRP MEEDGEEKSP SKKKKKIQKK EEKAEPPQAM NALMRLNQLK PGLQYKLVSQ421TGPVHAPIFT MSVEVDGNSF EASGPSKKTA KLHVAVKVLQ DMGLPTGAEG RDSSKGEDSA481EETEAKPAVV APAPVVEAVS TPSAAFPSDA TAEQGPILTK HGKNPVMELN EKRRGLKYEL541ISETGGSHDK RFVMEVEVDG QKFQGAGSNK KVAKAYAALA ALEKLFPDTP LALDANKKKR601APVPVRGGPK FAAKPHNPGF GMGGPMHNEV PPPPNLRGRG RGGSIRGRGR GRGFGGANHG661GYMNAGAGYG SYGYGGNSAT AGYSQFYSNG GHSGNASGGG GGGGGGSSGY GSYYQGDNYN721SPVPPKHAGK KQPHGGQQKP SYGSGYQSHQ GQQQSYNQSP YSNYGPPQGK QKGYNHGQGS781YSYSNSYNSP GGGGGSDYNY ESKFNYSGSG GRSGGNSYGS GGASYNPGSH GGYGGGSGGG841SSYQGKQGGY SQSNYNSPGS GQNYSGPPSS YQSSQGGYGR NADHSMNYQY RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
891 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.