Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NF2 / Merlin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127579-100
Catalog Number: LS-G127579
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NF2(NF2) (NM_181831), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACN | BANF | Moesin-ezrin-radixin like | Neurofibromin 2 (merlin) | Neurofibromin-2 | NF2 | SCH | Merlin | Schwannomerlin | Schwannomin
- Origin Species
- Human
- NCBI GenBank
- NM_000268
- SwissProt
- P35240
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGAIASRMS FSSLKRKQPK TFTVRIVTMD AEMEFNCEVK KQILDEKIYC PPEASVLLAS61YAVQAKYGDY DPSVHKRGFL AQEELLPKRV INLYQMTPEM WEERITAWYA EHRGRARDEA121EMEYLKIAQD LEMYGVNYFA IRNKKGTELL LGVDALGLHI YDPENRLTPK ISFPWNEIRN181ISYSDKEFTI KPLDKKIDVF KFNSSKLRVN KLILQLCIGN HDLFMRRRKA DSLEVQQMKA241QAREEKARKQ MERQRLAREK QMREEAERTR DELERRLLQM KEEATMANEA LMRSEETADL301LAEKAQITEE EAKLLAQKAA EAEQEMQRIK ATAIRTEEEK RLMEQKVLEA EVLALKMAEE361SERRAKEADQ LKQDLQEARE AERRAKQKLL EIATKPTYPP MNPIPAPLPP DIPSFNLIGD421SLSFDFKDTD MKRLSMEIEK EKVEYMEKSK HLQEQLNELK TEIEALKLKE RETALDILHN481ENSDRGGSSK HNTIKKPQAQ GRRPICITRTRPLEQKLISEEDLAANDILDYKDDDDKV
507 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.