LSBio
NEURL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NEURL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NEURL4 antibodies →
$725.00each
Catalog Number: LS-G108831-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEURL4 (NM_032442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032442

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
166.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGSGGSGG SGGGPGPGPG GGGGPSGSGS GPGSNGGLGS GGELHPRTGR LVSLSACGRT
61ARRQQPGQEF NHGLVLSREP LRDGRVFTVR IDRKVNSWSG SIEIGVTALD PSVLDFPSSA
121TGLKGGSWVV SGCSVLRDGR SVLEEYGQDL DQLGEGDRVG VERTVAGELR LWVNGRDCGV
181AATGLPPRVW AVVDLYGKCT QITVLPPEPG FSPPTPIPTP PLEPLAPTED SALAEQGTSA
241DEAFMVSPAQ ARPETFPNSL ESHNDFANME LSEVVSNTIL SAYNGGLLNV NLSSPPAGEG
301LGSSGAATSP ILTSNDALLF HEKCGTLIKL SNNNKTAERR RPLDEFNNGV VMTNRPLRDN
361EMFEIRIDKL VDKWSGSIEI GVTTHNPNSL EYPATMTNLQ SGTIMMSGCG ILTNGKGTRR
421EYCEFSLDEL QEGDHIGLTR KSNSALHFFI NGIDQGVATP LTPPVVYGVV DLYGMAVKVT
481IVHNNNHSDR LRRNNAILRA LSPEGALRRA APAAQAEPER LLFHPNCGQK AAITHEGRTA
541LRPHATDDFN HGVVLSSRAL RDGEVFQVRI DKMVDKWAGS IEIGVTTHNP AYLQLPSTMT
601NLRSGTWMMT GNGVMHNGTT ILDEYGHNLD RLKAGDTVGV VRREDGTLHF FVNGMTQGPA
661AWNVPPGVYA VVDLYGQAAQ ATIVDDVEVA PVPEPLPEGN NQVSPSSPSS GAGGSDLRFH
721QLHGSNAVIT NGGRTALRHN CRSEFNDAIV ISNRALRDGE LFEIVIQKMV DRWSGSIEAG
781VTAIRPEDLE FPNTMTDIDY DTWMLSGTAI MQDGNTMRNN YGCDLDALGT GARIGMMRTA
841KGDLHYFING QDQGAACSGL PPGKEVYAVV DLYGQCVQVS ITNATGPMDN SLATSNTATE
901KSFPLHSPVA GVAHRFHSTC GKNVTLEEDG TRAVRAAGYA HGLVFSTKEL RAEEVFEVKV
961EELDEKWAGS LRLGLTTLAP GEMGPGAGGG GPGLPPSLPE LRTKTTWMVS SCEVRRDGQL
1021QRMNYGRNLE RLGVGSRVGV RRGADDTMHI LVDGEDMGPA ATGIAKNVWA VLDLYGPVRG
1081VSIVSSTRLE ESEGTQPPSP SSDTGSEGEE DDEGEEHGLG GQNEVGIIPT TLEFLENHGK
1141NILLSNGNRT ATRVASYNQG IVVINQPLVP QLLVQVRIDF LNRQWTSSLV LGVITCAPER
1201LNFPASACAL KRAAWLLRGR GVFHNGLKIC EKFGPNLDTC PEGTILGLRL DSSGGLHLHV
1261NGVDQGVAVP DVPQPCHALV DLYGQCEQVT IVNPEPGAAS GKSAGTQGDM EKADMVDGIK
1321ESVCWGPPPA ASPLKSCEYH ALCSRFQELL LLPEDYFMPP PKRSLCYCES CRKLRGDEAH
1381RRRGEPPREY ALPFGWCRFN LRVNPRLEAG TLTKKWHMAY HGSNVAAVRR VLDRGELGAG
1441TASILSCRPL KGEPGVGFEE PGENCAPPRE EQPPPVLLSP SLQYAGAETL ASKVQFRDPK
1501SQRTHQAQVA FQVCVRPGSY TPGPPSAALG EPPDPHFSPA ELEWVTKEKG ATLLCALLVR
1561VE
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1562 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.