Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NEURL1 / NEURL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116796-100
Catalog Number: LS-G116796
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEURL(NEURL1) (NM_004210), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- H-neuralized 1 | Neu | Neu-1 | Neuralized-like (Drosophila) | Neuralized-like protein 1A | NEURL | NEURL1 | RNF67 | RING finger protein 67 | BA416N2.1 | H-neu | Neuralized (Drosophila)-like | NEURL1A
- Origin Species
- Human
- NCBI GenBank
- NM_004210
- SwissProt
- O76050
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNNFSSIPS LPRGNPSRAP RGHPQNLKDS IGGPFPVTSH RCHHKQKHCP AVLPSGGLPA61TPLLFHPHTK GSQILMDLSH KAVKRQASFC NAITFSNRPV LIYEQVRLKI TKKQCCWSGA121LRLGFTSKDP SRIHPDSLPK YACPDLVSQS GFWAKALPEE FANEGNIIAF WVDKKGRVFH181RINDSAVMLF FSGVRTADPL WALVDVYGLT RGVQLLDSEL VLPDCLRPRS FTALRRPSLR241READDARLSV SLCDLNVPGA DGDEAAPAAG CPIPQNSLNS QHSRALPAQL DGDLRFHALR301AGAHVRILDE QTVARVEHGR DERALVFTSR PVRVAETIFV KVTRSGGARP GALSFGVTTC361DPGTLRPADL PFSPEALVDR KEFWAVCRVP GPLHSGDILG LVVNADGELH LSHNGAAAGM421QLCVDASQPL WMLFGLHGTI TQIRILGSTI LAERGIPSLP CSPASTPTSP SALGSRLSDP481LLSTCSSGPL GSSAGGTAPN SPVSLPESPV TPGLGQWSDE CTICYEHAVD TVIYTCGHMC541LCYACGLRLK KALHACCPIC RRPIKDIIKT YRSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
574 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.