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NEU4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NEU4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NEU4 antibodies →
$504.00each
Catalog Number: LS-G107764-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEU4 (NM_080741), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
N-acetyl-alpha-neuraminidase 4 | NEU4 | Sialidase 4 | Sialidase-4 | Neuraminidase 4
Origin Species
Human
SwissProt
Q8WWR8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSSAAFPRW LSMGVPRTPS RTVLFERERT GLTYRVPSLL PVPPGPTLLA FVEQRLSPDD
61SHAHRLVLRR GTLAGGSVRW GALHVLGTAA LAEHRSMNPC PVHDAGTGTV FLFFIAVLGH
121TPEAVQIATG RNAARLCCVA SRDAGLSWGS ARDLTEEAIG GAVQDWATFA VGPGHGVQLP
181SGRLLVPAYT YRVDRRECFG KICRTSPHSF AFYSDDHGRT WRCGGLVPNL RSGECQLAAV
241DGGQAGSFLY CNARSPLGSR VQALSTDEGT SFLPAERVAS LPETAWGCQG SIVGFPAPAP
301NRPRDDSWSV GPGSPLQPPL LGPGVHEPPE EAAVDPRGGQ VPGGPFSRLQ PRGDGPRQPG
361PRPGVSGDVG SWTLALPMPF AAPPQSPTWL LYSHPVGRRA RLHMGIRLSQ SPLDPRSWTE
421PWVIYEGPSG YSDLASIGPA PEGGLVFACL YESGARTSYD EISFCTFSLR EVLENVPASP
481KPPNLGDKPR GCCWPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.