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NELL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NELL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126529-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NELL2 (NM_001145110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NEL-like 2 (chicken) | NEL-like protein 2 | Nel (chicken)-like 2 | Nel-related protein 2 | NELL2
Origin Species
Human
NCBI GenBank
NM_006159
SwissProt
Q99435

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSIRRLLILI LKIGRRWTEL IRTMESRVLL RTFCLIFGLG AVWGLGVDPS LQIDVLTELE
61LGESTTGVRQ VPGLHNGTKA FLFQDTPRSI KASTATAEQF FQKLRNKHEF TILVTLKQTH
121LNSGVILSIH HLDHRYLELE SSGHRNEVRL HYRSGSHRPH TEVFPYILAD DKWHKLSLAI
181SASHLILHID CNKIYERVVE KPSTDLPLGT TFWLGQRNNA HGYFKGIMQD VQLLVMPQGF
241IAQCPDLNRT CPTCNDFHGL VQKIMELQDI LAKTSAKLSR AEQRMNRLDQ CYCERTCTMK
301GTTYREFESW IDGCKNCTCL NGTIQCETLI CPNPDCPLKS ALAYVDGKCC KECKSICQFQ
361GRTYFEGERN TVYSSSGVCV LYECKDQTMK LVESSGCPAL DCPESHQITL SHSCCKVCKG
421YDFCSERHNC MENSICRNLN DRAVCSCRDG FRALREDNAY CEDIDECAEG RHYCRENTMC
481VNTPGSFMCI CKTGYIRIDD YSCTEHDECI TNQHNCDENA LCFNTVGGHN CVCKPGYTGN
541GTTCKAFCKD GCRNGGACIA ANVCACPQGF TGPSCETDID ECSDGFVQCD SRANCINLPG
601WYHCECRDGY HDNGMFSPSG ESCEDIDECG TGRHSCANDT ICFNLDGGYD CRCPHGKNCT
661GDCIHDGKVK HNGQIWVLEN DRCSVCSCQN GFVMCRRMVC DCENPTVDLF CCPECDPRLS
721SQCLHQNGET LYNSGDTWVQ NCQQCRCLQG EVDCWPLPCP DVECEFSILP ENECCPRCVT
781DPCQADTIRN DITKTCLDEM NVVRFTGSSW IKHGTECTLC QCKNGHICCS VDPQCLQEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.