Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NEFH / NF-H Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110788-100
Catalog Number: LS-G110788
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFH(NEFH) (NM_021076), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H | NEFH
- Origin Species
- Human
- NCBI GenBank
- NM_021076
- SwissProt
- P12036
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMSFGGADAL LGAPFAPLHG GGSLHYALAR KGGAGGTRSA AGSSSGFHSW TRTSVSSVSA61SPSRFRGAGA ASSTDSLDTL SNGPEGCMVA VATSRSEKEQ LQALNDRFAG YIDKVRQLEA121HNRSLEGEAA ALRQQQAGRS AMGELYEREV REMRGAVLRL GAARGQLRLE QEHLLEDIAH181VRQRLDDEAR QREEAEAAAR ALARFAQEAE AARVDLQKKA QALQEECGYL RRXHQEEVGE241LLGQIQGSGA AQAQMQAETR DALKCDVTSA LREIRAQLEG HAVQSTLQSE EWFRVRLDRL301SEAAKVNTDA MRSAQEEITE YRRQLQARTT ELEALKSTKD SLERQRSELE DRHQADIASY361QEAIQQLDAE LRNTKWEMAA QLREYQDLLN VKMALDIEIA AYRKLLEGEE CRIGFGPIPF421SLPEGLPKIP SVSTHIKVKS EEKIKVVEKS EKETVIVEEQ TEETQVTEEV TEEEEKEAKE481EEGKEEEGGE EEEAEGGEEE TKSPPAEEAA SPEKEAKSPV KEEAKSPAEA KSPEKEEAKS541PAEVKSPEKA KSPAKEEAKS PPEAKSPEKE EAKSPAEVKS PEKAKSPAKE EAKSPAEAKS601PEKAKSPVKE EAKSLAEAKS PVKEEAKSPA EVKSPEKAKS PTKEEAKSPE KAKSPEKEEA661KSPEKAKSPV KAEAKSPEKA KSPVKAEAKS PEKAKSPVKE EAKSPEKAKS PVKEEAKSPE721KAKSPVKEEA KTPEKAKSPV KEEAKSPEKA KSPEKAKTLD VKSPEAKTPA KEEARSPADK781FPEKAKSPVK EEVKSPEKAK SPLKEDAKAP EKEIPKKEEV KSPVKEEEKP QEVKVKEPPK841KAEEEKAPAT PKTEEKKDSK KEEAPKKEAP KPKVEEKKEP AVEKPKESKV EAKKEEAEDK901KKVPTPEKEA PAKVEVKEDA KPKEKTEVAK KEPDDAKAKE PSKPAEKKEA APEKKDTKEE961KAKKPEEKPK TEAKAKEDDK TLSKEPSKPK AEKAEKSSST DQKDSKPPEK ATEDKAAKGKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1020 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.