Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NEDD4L / NEDD4-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126444-100
Catalog Number: LS-G126444
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEDD4 2(NEDD4L) (NM_001144964), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HNEDD4-2 | KIAA0439 | NEDD4-2 | NEDD4L | RSP5 | NEDD4.2 | NEDL3 | Ubiquitin-protein ligase Rsp5
- Origin Species
- Human
- NCBI GenBank
- AB007899
- SwissProt
- Q96PU5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MERPYTFKDF LLRPRSHKSR VKGFLRLKMA YMPKNGGQDE ENSDQRDDME HGWEVVDSND61SASQHQEELP PPPLPPGWEE KVDNLGRTYY VNHNNRTTQW HRPSLMDVSS ESDNNIRQIN121QEAAHRRFRS RRHISEDLEP EPSEGGDVPE PWETISEEVN IAGDSLGLAL PPPPASPGSR181TSPQELSEEL SRRLQITPDS NGEQFSSLIQ REPSSRLRSC SVTDAVAEQG HLPPPSAPAG241RARSSTVTGG EEPTPSVAYV HTTPGLPSGW EERKDAKGRT YYVNHNNRTT TWTRPIMQLA301EDGASGSATN SNNHLIEPQI RRPRSLSSPT VTLSAPLEGA KDSPVRRAVK DTLSNPQSPQ361PSPYNSPKPQ HKVTQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSKTS421LNPNDLGPLP PGWEERIHLD GRTFYIDHNS KITQWEDPRL QNPAITGPAV PYSREFKQKY481DYFRKKLKKP ADIPNRFEMK LHRNNIFEES YRRIMSVKRP DVLKARLWIE FESEKGLDYG541GVAREWFFLL SKEMFNPYYG LFEYSATDNY TLQINPNSGL CNEDHLSYFT FIGRVAGLAV601FHGKLLDGFF IRPFYKMMLG KQITLNDMES VDSEYYNSLK WILENDPTEL DLMFCIDEEN661FGQTYQVDLK PNGSEIMVTN ENKREYIDLV IQWRFVNRVQ KQMNAFLEGF TELLPIDLIK721IFDENELELL MCGLGDVDVN DWRQHSIYKN GYCPNHPVIQ WFWKAVLLMD AEKRIRLLQF781VTGTSRVPMN GFAELYGSNG PQLFTIEQWG SPEKLPRAHT CFNRLDLPPY ETFEDLREKL841LMAVENAQGF EGVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
854 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.