Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NECL-1 / CADM3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110715-100
Catalog Number: LS-G110715
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CADM3 (NM_021189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain immunoglobulin receptor | CADM3 | Cell adhesion molecule 3 | BIgR | Nectin-like 1 | IGSF4B | SynCAM3 | TSLC1-like protein 1 | NECL1 | Nectin-like protein 1 | TSLL1 | Necl-1 | TSLC1-like 1
- Origin Species
- Human
- NCBI GenBank
- NM_021189
- SwissProt
- Q8N126
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAPAASLLL LLLLFACCWA PGGANLSQDG YWQEQDLELG TLAPLDEAIS STVWSSPDML61ASQDSQPWTS DETVVAGGTV VLKCQVKDHE DSSLQWSNPA QQTLYFGEKR ALRDNRIQLV121TSTPHELSIS ISNVALADEG EYTCSIFTMP VRTAKSLVTV LGIPQKPIIT GYKSSLREKD181TATLNCQSSG SKPAARLTWR KGDQELHGEP TRIQEDPNGK TFTVSSSVTF QVTREDDGAS241IVCSVNHESL KGADRSTSQR IEVLYTPTAM IRPDPPHPRE GQKLLLHCEG RGNPVPQQYL301WEKEGSVPPL KMTQESALIF PFLNKSDSGT YGCTATSNMG SYKAYYTLNV NDPSPVPSSS361STYHAIIGGI VAFIVFLLLI MLIFLGHYLI RHKGTYLTHE AKGSDDAPDA DTAIINAEGG421QSGGDDKKEY FITRTRPLEQKLISEEDLAANDILDYKDDDDKV
432 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.