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NDST3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NDST3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G100389-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NDST3 (NM_004784), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HNDST-3 | NDST-3 | NDST3 | N-HSST 3 | HSST3
Origin Species
Human
NCBI GenBank
NM_004784
SwissProt
O95803

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFIMKLHRH FQRTVILLAT FCMVSIIISA YYLYSGYKQE NELSETASEV DCGDLQHLPY
61QLMEVKAMKL FDASRTDPTV LVFVESQYSS LGQDIIMILE SSRFQYHIEI APGKGDLPVL
121IDKMKGKYIL IIYENILKYI NMDSWNRSLL DKYCVEYGVG VIGFHKTSEK SVQSFQLKGF
181PFSIYGNLAV KDCCINPHSP LIRVTKSSKL EKGSLPGTDW TVFQINHSAY QPVIFAKVKT
241PENLSPSISK GAFYATIIHD LGLHDGIQRV LFGNNLNFWL HKLIFIDAIS FLSGKRLTLS
301LDRYILVDID DIFVGKEGTR MNTNDVKALL DTQNLLRAQI TNFTFNLGFS GKFYHTGTEE
361EDEGDDCLLG SVDEFWCFPH MWSHMQPHLF HNESSLVEQM ILNKKFALEH GIPTDMGYAV
421APHHSGVYPV HVQLYEAWKK VWNIKITSTE EYPHLKPARY RRGFIHKNIM VLPRQTCGLF
481THTIFYKEYP GGPKELDKSI QGGELFFTVV LNPISIFMTH LSNYGNDRLG LYTFVNLANF
541VKSWTNLRLQ TLPPVQLAHK YFELFPDQKD PLWQNPCDDK RHRDIWSKEK TCDRLPKFLV
601IGPQKTGTTA LYLFLVMHPS ILSNSPSPKT FEEVQFFNRN NYHRGIDWYM DFFPVPSNVT
661TDFLFEKSAN YFHSEEAPKR AASLVPKAKI ITILIDPSDR AYSWYQHQRS HEDPAALKFS
721FYEVISAGPR APSELRALQK RCLVPGWYAS HIERWLVYFP PFQLLIIDGQ QLRTDPATVM
781DEVQKFLGVL PHYNYSEALT FDSHKGFWCQ LLEEGKTKCL GKSKGRKYPP MDSDSRTFLS
841SYYRDHNVEL SKLLHKLGQP LPSWLRQELQ KVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

873 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.