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NDRG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NDRG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112767-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NDRG2 (NM_016250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1248 | N-myc downstream regulator 2 | NDRG family member 2 | NDRG2 | SYLD | NDR1-related protein NDR2 | Protein Syld709613 | Syld709613 protein | Cytoplasmic protein Ndr1 | Protein NDRG2
Origin Species
Human
NCBI GenBank
NM_016250
SwissProt
Q9UN36

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELQEVQIT EEKPLLPGQT PEAAKTHSVE TPYGSVTFTV YGTPKPKRPA ILTYHDVGLN
61YKSCFQPLFQ FEDMQEIIQN FVRVHVDAPG MEEGAPVFPL GYQYPSLDEL ADMIPCVLQY
121LNFSTIIGVG VGAGAYILAR YALNHPDTVE GLVLINIDPN AKGWMDWAAH KLTGLTSSIP
181EMILGHLFSQ EELSGNSELI QKYRNIITHA PNLDNIELYW NSYNNRRDLN FERGGDITLR
241CPVMLVVGDQ APHEDAVVEC NSKLDPTRTS FLKMADSGGQ PQLTQPGKLT EAFKYFLQGM
301GYMASSCMTR LSRSRTASLT SAASVDGNRS RSRTLSQSSE SGTLSSGPPG HTMEVSC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

357 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.