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NDNF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NDNF Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NDNF antibodies →
$504.00each
Catalog Number: LS-G101888-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NDNF (NM_024574), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C4orf31 | Protein NDNF | NDNF
Origin Species
Human
NCBI GenBank
NM_024574
SwissProt
Q8TB73

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLLHWCLLW LLFPLSSRTQ KLPTRDEELF QMQIRDKAFF HDSSVIPDGA EISSYLFRDT
61PKRYFFVVEE DNTPLSVTVT PCDAPLEWKL SLQELPEDRS GEGSGDLEPL EQQKQQIINE
121EGTELFSYKG NDVEYFISSS SPSGLYQLDL LSTEKDTHFK VYATTTPESD QPYPELPYDP
181RVDVTSLGRT TVTLAWKPSP TASLLKQPIQ YCVVINKEHN FKSLCAVEAK LSADDAFMMA
241PKPGLDFSPF DFAHFGFPSD NSGKERSFQA KPSPKLGRHV YSRPKVDIQK ICIGNKNIFT
301VSDLKPDTQY YFDVFVVNIN SNMSTAYVGT FARTKEEAKQ KTVELKDGKI TDVFVKRKGA
361KFLRFAPVSS HQKVTFFIHS CLDAVQIQVR RDGKLLLSQN VEGIQQFQLR GKPKAKYLVR
421LKGNKKGASM LKILATTRPT KQSFPSLPED TRIKAFDKLR TCSSATVAWL GTQERNKFCI
481YKKEVDDNYN EDQKKREQNQ CLGPDIRKKS EKVLCKYFHS QNLQKAVTTE TIKGLQPGKS
541YLLDVYVIGH GGHSVKYQSK VVKTRKFC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

568 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.