Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NCR1 / NKP46 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116379-100
Catalog Number: LS-G116379
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCR1 (NM_004829), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD335 | HNKp46 | NCR1 | NK-p46 | Lymphocyte antigen 94 homolog | NK cell-activating receptor | CD335 antigen | LY94 | NKP46
- Origin Species
- Human
- NCBI GenBank
- NM_004829
- SwissProt
- O76036
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSTLPALLC VGLCLSQRIS AQQQTLPKPF IWAEPHFMVP KEKQVTICCQ GNYGAVEYQL61HFEGSLFAVD RPKPPERINK VKFYIPDMNS RMAGQYSCIY RVGELWSEPS NLLDLVVTEM121YDTPTLSVHP GPEVISGEKV TFYCRLDTAT SMFLLLKEGR SSHVQRGYGK VQAEFPLGPV181TTAHRGTYRC FGSYNNHAWS FPSEPVKLLV TGDIENTSLA PEDPTFPDTW GTYLLTTETG241LQKDHALWDH TAQNLLRMGL AFLVLVALVW FLVEDWLSRK RTRERASRAS TWEGRRRLNT301QTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
303 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.