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NCOA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NCOA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NCOA7 antibodies →
$725.00each
Catalog Number: LS-G104373-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCOA7 (NM_181782), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ187J11.3 | ERAP140 | Nbla00052 | Nbla10993 | ESNA1 | NCOA7 | Nuclear receptor coactivator 7
Origin Species
Human
SwissProt
Q8NI08

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDTKEEKKER KQSYFARLKK KKQAKQNAET ASAVATRTHT GKEDNNTVVL EPDKCNIAVE
61EEYMTDEKKK RKSNQLKEIR RTELKRYYSI DDNQNKTHDK KEKKMVVQKP HGTMEYTAGN
121QDTLNSIALK FNITPNKLVE LNKLFTHTIV PGQVLFVPDA NSPSSTLRLS SSSPGATVSP
181SSSDAEYDKL PDADLARKAL KPIERVLSST SEEDEPGVVK FLKMNCRYFT DGKGVVGGVM
241IVTPNNIMFD PHKSDPLVIE NGCEEYGLIC PMEEVVSIAL YNDISHMKIK DALPSDLPQD
301LCPLYRPGEW EDLASEKDIN PFSKFKSINK EKRQQNGEKI MTSDSRPIVP LEKSTGHTPT
361KPSGSSVSEK LKKLDSSRET SHGSPTVTKL SKEPSDTSSA FESTAKENFL GEDDDFVDLE
421ELSSQTGGGM HKKDTLKECL SLDPEERKKA ESQINNSAVE MQVQSALAFL GTENDVELKG
481ALDLETCEKQ DIMPEVDKQS GSPESRVENT LNIHEDLDKV KLIEYYLTKN KEGPQVSENL
541QKTELSDGKS IEPGGIDITL SSSLSQAGDP ITEGNKEPDK TWVKKGEPLP VKLNSSTEAN
601VIKEALDSSL ESTLDNSCQG AQMDNKSEVQ LWLLKRIQVP IEDILPSKEE KSKTPPMFLC
661IKVGKPMRKS FATHTAAMVQ QYGKRRKQPE YWFAVPRERV DHLYTFFVQW SPDVYGKDAK
721EQGFVVVEKE ELNMIDNFFS EPTTKSWEII TVEEAKRRKS TCSYYEDEDE EVLPVLRPHS
781ALLENMHIEQ LARRLPARVQ GYPWRLAYST LEHGTSLKTL YRKSASLDSP VLLVIKDMDN
841QIFGAYATHP FKFSDHYYGT GETFLYTFSP HFKVFKWSGE NSYFINGDIS SLELGGGGGR
901FGLWLDADLY HGRSNSCSTF NNDILSKKED FIVQDLEVWA FD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

942 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.