Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NCF2 / NOXA2 / p67phox Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130960-100
Catalog Number: LS-G130960
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCF2(NCF2) (NM_001190789), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NADPH oxidase activator 2 | Neutrophil cytosol factor 2 | Neutrophil cytosolic factor 2 | NOXA2 | p67PHOX | NCF-2 | NCF2 | p67-PHOX
- Origin Species
- Human
- NCBI GenBank
- NM_000433
- SwissProt
- P19878
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLVEAISLW NEGVLAADKK DWKGALDAFS AVQDPHSRIC FNIGCMYTIL KNMTEAEKAF61TRSINRDKHL AVAYFQRGML YYQTEKYDLA IKDLKEALIQ LRGNQLIDYK ILGLQFKLFA121CEVVASVVDQ DSFSGFAPLQ PQAAEPPPRP KTPEIFRALE GEAHRVLFGF VPETKEELQV181MPGNIVFVLK KGNDNWATVM FNGQKGLVPC NYLEPVELRI HPQQQPQEES SPQSDIPAPP241SSKAPGRPQL SPGQKQKEEP KEVKLSVPMP YTLKVHYKYT VVMKTQPGLP YSQVRDMVSK301KLELRLEHTK LSYRPRDSNE LVPLSEDSMK DAWGQVKNYC LTLWCENTVG DQGFPDEPKE361SEKADANNQT TEPQLKKGSQ VEALFSYEAT QPEDLEFQEG DIILVLSKVN EEWLEGECKG421KVGIFPKVFV EDCATTDLES TRREVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.