Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NCEH1 / AADACL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110987-100
Catalog Number: LS-G110987
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AADACL1(NCEH1) (NM_020792), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AADACL1 | KIAA1363 | NCEH | NCEH1
- Origin Species
- Human
- NCBI GenBank
- AB037784
- SwissProt
- Q6PIU2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSCRGQKVA GGLRVVSPFP LCQPAGEPSR GKMRSSCVLL TALVALAAYY VYIPLPGSVS61DPWKLMLLDA TFRGAQQVSN LIHYLGLSHH LLALNFIIVS FGKKSAWSSA QVKVTDTDFD121GVEVRVFEGP PKPEEPLKRS VVYIHGGGWA LASAKIRYYD ELCTAMAEEL NAVIVSIEYR181LVPKVYFPEQ IHDVVRATKY FLKPEVLQKY MVDPGRICIS GDSAGGNLAA ALGQQFTQDA241SLKNKLKLQA LIYPVLQALD FNTPSYQQNV NTPILPRYVM VKYWVDYFKG NYDFVQAMIV301NNHTSLDVEE AAAVRARLNW TSLLPASFTK NYKPVVQTTG NARIVQELPQ LLDARSAPLI361ADQAVLQLLP KTYILTCEHD VLRDDGIMYA KRLESAGVEV TLDHFEDGFH GCMIFTSWPT421NFSVGIRTRN SYIKWLDQNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.