Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NCAPH2 / CAP-H2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113894-100
Catalog Number: LS-G113894
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCAPH2 (NM_014551), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 384D8-2 | CAPH2 | CAP-H2 | HCAP-H2 | NCAPH2 | Kleisin beta | Condensin-2 complex subunit H2 | Kleisin-beta
- Origin Species
- Human
- NCBI GenBank
- NM_014551
- SwissProt
- Q6IBW4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDVEARFAH LLQPIRDLTK NWEVDVAAQL GEYLEELDQI CISFDEGKTT MNFIEAALLI61QGSACVYSKK VEYLYSLVYQ ALDFISGKRR AKQLSSVQED RANGVASSGV PQEAENEFLS121LDDFPDSRTN VDLKNDQTPS EVLIIPLLPM ALVAPDEMEK NNNPLYSRQG EVLASRKDFR181MNTCVPHPRG AFMLEPEGMS PMEPAGVSPM PGTQKDTGRT EEQPMEVSVC RSPVPALGFS241QEPGPSPEGP MPLGGGEDED AEEAVELPEA SAPKAALEPK ESRSPQQVGP TWRPAEPELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
299 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.