NCAPD3 / HCAP-D3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113322-100
Catalog Number: LS-G113322
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human hCAP D3(NCAPD3) (NM_015261), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAP-D3 | CAPD3 | Condensin-2 complex subunit D3 | HHCP-6 | HCAP-D3 | KIAA0056 | NCAPD3 | Hcp-6
- Origin Species
- Human
- NCBI GenBank
- D29954
- SwissProt
- P42695
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 168.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVALRGLGSG LQPWCPLDLR LEWVDTVWEL DFTETEPLDP SIEAEIIETG LAAFTKLYES61LLPFATGEHG SMESIWTFFI ENNVSHSTLV ALFYHFVQIV HKKNVSVQYR EYGLHAAGLY121FLLLEVPGSV ANQVFHPVMF DKCIQTLKKS WPQESNLNRK RKKEQPKSSQ ANPGRHRKRG181KPPRREDIEM DEIIEEQEDE NICFSARDLS QIRNAIFHLL KNFLRLLPKF SLKEKPQCVQ241NCIEVFVSLT NFEPVLHECH VTQARALNQA KYIPELAYYG LYLLCSPIHG EGDKVISCVF301HQMLSVILML EVGEGSHRAP LAVTSQVINC RNQAVQFISA LVDELKESIF PVVRILLQHI361CAKVVDKSEY RTFAAQSLVQ LLSKLPCGEY AMFIAWLYKY SRSSKIPHRV FTLDVVLALL421ELPEREVDNT LSLEHQKFLK HKFLVQEIMF DRCLDKAPTV RSKALSSFAH CLELTVTSAS481ESILELLINS PTFSVIESHP GTLLRNSSAF SYQRQTSNRS EPSGEINIDS SGETVGSGER541CVMAMLRRRI RDEKTNVRKS ALQVLVSILK HCDVSGMKED LWILQDQCRD PAVSVRKQAL601QSLTELLMAQ PRCVQIQKAW LRGVVPVVMD CESTVQEKAL EFLDQLLLQN IRHHSHFHSG661DDSQVLAWAL LTLLTTESQE LSRYLNKAFH IWSKKEKFSP TFINNVISHT GTEHSAPAWM721LLSKIAGSSP RLDYSRIIQS WEKISSQQNP NSNTLGHILC VIGHIAKHLP KSTRDKVTDA781VKCKLNGFQW SLEVISSAVD ALQRLCRASA ETPAEEQELL TQVCGDVLST CEHRLSNIVL841KENGTGNMDE DLLVKYIFTL GDIAQLCPAR VEKRIFLLIQ SVLASSADAD HSPSSQGSSE901APASQPPPQV RGSVMPSVIR AHAIITLGKL CLQHEDLAKK SIPALVRELE VCEDVAVRNN961VIIVMCDLCI RYTIMVDKYI PNISMCLKDS DPFIRKQTLI LLTNLLQEEF VKWKGSLFFR1021FVSTLIDSHP DIASFGEFCL AHLLLKRNPV MFFQHFIECI FHFNNYEKHE KYNKFPQSER1081EKRLFSLKGK SNKERRMKIY KFLLEHFTDE QRFNITSKIC LSILACFADG ILPLDLDASE1141LLSDTFEVLS SKEIKLLAMR SKPDKDLLME EDDMALANVV MQEAQKKLIS QVQKRNFIEN1201IIPIIISLKT VLEKNKIPAL RELMHYLREV MQDYRDELKD FFAVDKQLAS ELEYDMKKYQ1261EQLVQEQELA KHADVAGTAG GAEVAPVAQV ALCLETVPVP AGQENPAMSP AVSQPCTPRA1321SAGHVAVSSP TPETGPLQRL LPKARPMSLS TIAILNSVKK AVESKSRHRS RSLGVLPFTL1381NSGSPEKTCS QVSSYSLEQE SNGEIEHVTK RAISTPEKSI SDVTFGAGVS YIGTPRTPSS1441AKEKIEGRSQ GNDILCLSLP DKPPPQPQQW NVRSPARNKD TPACSRRSLR KTPLKTANTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1498 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.