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NBR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NBR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NBR1 antibodies →
$725.00each
Catalog Number: LS-G109158-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NBR1 (NM_031858), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
1A1-3B | 1A13B | CA125 | M17S2 | MIG19 | NBR1 | Migration-inducing protein 19 | Neighbor of BRCA1 gene 1 | KIAA0049 | Next to BRCA1 gene 1 protein | Protein 1A1-3B
Origin Species
Human
NCBI GenBank
NM_005899
SwissProt
Q14596

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPQVTLNVT FKNEIQSFLV SDPENTTWAD IEAMVKVSFD LNTIQIKYLD EENEEVSINS
61QGEYEEALKM AVKQGNQLQM QVHEGHHVVD EAPPPVVGAK RLAARAGKKP LAHYSSLVRV
121LGSDMKTPED PAVQSFPLVP CDTDQPQDKP PDWFTSYLET FREQVVNETV EKLEQKLHEK
181LVLQNPSLGS CPSEVSMPTS EETLFLPENQ FSWHIACNNC QRRIVGVRYQ CSLCPSYNIC
241EDCEAGPYGH DTNHVLLKLR RPVVGSSEPF CHSKYSTPRL PAALEQVRLQ KQVDKNFLKA
301EKQRLRAEKK QRKAEVKELK KQLKLHRKIH LWNSIHGLQS PKSPLGRPES LLQSNTLMLP
361LQPCTSVMPM LSAAFVDENL PDGTHLQPGT KFIKHWRMKN TGNVKWSADT KLKFMWGNLT
421LASTEKKDVL VPCLKAGHVG VVSVEFIAPA LEGTYTSHWR LSHKGQQFGP RVWCSIIVDP
481FPSEESPDNI EKGMISSSKT DDLTCQQEET FLLAKEERQL GEVTEQTEGT AACIPQKAKN
541VASERELYIP SVDLLTAQDL LSFELLDINI VQELERVPHN TPVDVTPCMS PLPHDSPLIE
601KPGLGQIEEE NEGAGFKALP DSMVSVKRKA ENIASVEEAE EDLSGTQFVC ETVIRSLTLD
661AAPDHNPPCR QKSLQMTFAL PEGPLGNEKE EIIHIAEEEA VMEEEEDEED EEEEDELKDE
721VQSQSSASSE DYIIILPECF DTSRPLGDSM YSSALSQPGL ERGAEGKPGV EAGQEPAEAG
781ERLPGGENQP QEHSISDILT TSQTLETVPL IPEVVELPPS LPRSSPCVHH HGSPGVDLPV
841TIPEVSSVPD QIRGEPRGSS GLVNSRQKSY DHSRHHHGSS IAGGLVKGAL SVAASAYKAL
901FAGPPVTAQP IISEDQTAAL MARLFEMGFC DRQLNLRLLK KHNYNILQVV TELLQLNNND
961WYSQRY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

966 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.