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NBPF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NBPF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NBPF4 antibodies →
$504.00each
Catalog Number: LS-G126283-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NBPF4 (NM_001143989), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVSADPLSS ERAEMNILEI NQELRSQLAE SNQQFRDLKE KFLITQATAY SLANQLKKYK
61CEEYKDIIDS VLRDELQSME KLAEKLRQAE ELRQYKALVH SQAKELTQLR EKLREGRDAS
121RWLNKHLKTL LTPDDPDKSQ GQDLREQLAE GHRLAEHLVH KLSPENDEDE DEDEDDKDEE
181VEKVQESPAP REVQKTEEKE VPQDSLEECA VTCSNSHNPS NSNQPHRSTK ITFKEHEVDS
241ALVVESEHPH DEEEEALNIP PENQNDHEEE EGKAPVPPRH HDKSNSYRHR EVSFLALDEQ
301KVCSAQDVAR DYSNPKWDET SLGFLEKQSD LEEVKGQETV APRLSRGPLR VDKHEIPQES
361LDGCCLTPSI LPDLTPSYHP YWSTLYSFED KQVSLALVDK IKKDQEEIED QSPPCPRLSQ
421ELPEVKEQEV PEDSVNEVYL TPSVHHDVSD CHQPYSSTLS SLEDQLACSA LDVASPTEAA
481CPQGTWSGDL SHHQSEVQVS QAQLEPSTLV PSCLRLQLDQ GFHCGNGLAQ RGLSSTTCSF
541SANADSGNQW PFQELVLEPS LGMKNPPQLE DDALEGSASN TQGRQVTGRI RASLVLILKT
601IRRRLPFSKW RLAFRFAGPH AESAEIPNTA GRTQRMAG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

638 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.