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NBEAL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NBEAL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113433-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NBEAL2 (NM_015175), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BDPLT4 | KIAA0540 | GPS | NBEAL2 | Neurobeachin-like protein 2 | Neurobeachin-like 2
Origin Species
Human
NCBI GenBank
AB011112
SwissProt
Q6ZNJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
302.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASERLYEL WLLYYAQKDL GYLQQWLKAF VGAFKKSISL SSLEPRRPEE AGAEVPLLPL
61DELHVLAEQL HQADLEQALL LLKLFIILCR NLENIEAGRG QVLVPRVLAL LTKLVAELKG
121CPPPQGRGTQ LENVALHALL LCEGLFDPYQ TWRRQRSGEV ISSKEKSKYK FPPAALPQEF
181SAFFQESLQN ADHLPPILLL RLIHLFCAVL AGGKENGQMA VSDGSVKGLL SVVRGWSRGP
241APDPCLVPLA LEALVGAVHV LHASRAPPRG PELRALLESY FHVLNADWPA GLSSGPEEAL
301VTLRVSMLDA IPMMLACEDR PVLQATFLSN NCFEHLTRLI QNSKLYLQSR APPEGDSDLA
361TRLLTEPDVQ KVLDQDTDAI AVHVVRVLTC IMSDSPSAKE VFKERIGYPH LQEVLQSHGP
421PTHRLLQELL NMAVEGDHSM CPPPPIRNEQ PVLVLAQWLP SLPTAELRLF LAQRLRWLCD
481SCPASRATCV QAGLVGCLLE TLSTGLALEA RCQEQLLALL QALGRVSIRP MELRHLLRPR
541PGLDSEPGGA EAGKARHAGA VIRTLSGMAR HQGPARALRY FDLTPSMAGI MVPPVQRWPG
601PGFTFHAWLC LHPMDTAPTP APTRPLQRKQ LYSFFTSSGS GFEAFFTAAG TLVVAVCTRK
661EYLTMSLPEV SFADSAWHCV AIVHVPGRRP FSQNLVHVYK DGHLVKTAPL RCPSLSEPFS
721SCCIGSAGYR TTTTTTGLPT PPVPATLAYT HPALTRSQSV PASTGLGWGS GLVAPLQEGS
781IDSTLAGTQD TRWGSPTSLE GELGAVAIFH EALQATALRT LCTLGPNETA PFKPEGELHE
841LSTRLLLHYS PQACKNNICL DLSPSHGLDG RLTGHRVETW DVKDVVNCVG GMGALLPLLE
901RVAAQPKEAE AGPAETHDLV GPELTSGHNT QGLVLPLGKS SEERMERNAV AAFLLMLRNF
961LQGHMVNQES LVQCQGPAII GALLRKVPSW AMDMNVLMSA QLLMEQVAAE GSGPLLYLLY
1021QHLLFNFHLW TLSDFAVRLG HIQYMSSIVR EHRQKLRKKY GVQFILDALR THYSPQRERP
1081LAADDLRTVQ TSLLGLAREF LVRSLSADDV QVTQTMLSFL AATGDDGQAV GALDLLLALL
1141HGSLVQESLA VFLLEPGNLE VLLALLVRPG SLPLLPDRVC KILRRLQQNE RLPERSRQRL
1201RLRECGLQGL VACLPEGTVS PQLCQGLYKL FLGADCLNLS DLLAVVQLSL QADLSVRLDI
1261CRQLFHLIYG QPDVVRLLAR QAGWQDVLTR LYVLEAATAG SPPPSSPESP TSPKPAPPKP
1321PTESPAEPSD VFLPSEAPCP DPDGFYHALS PFCTPFDLGL ERSSVGSGNT AGGGGSSGTL
1381TPASQPGTPS PLDGPRPFPA APGRHSSSLS NVLEDGSLPE PTISGDDTSN TSNPQQTSEE
1441ELCNLLTNVL FSVTWRGVEG SDEAAWRERG QVFSVLTQLG ASATLVRPPD CIKRSLLEMM
1501LESALTDIKE APVGVLASLT QQALWLLRLL QDFLCAEGHG NQELWSEKLF EGVCSLLDRL
1561GAWPHLANGT ADLREMAQIG LRLVLGYILL EDPQLHAQAY VRLHMLLQTA VPARREEACY
1621VLSKLEAALG RVLNTSSLES ATDEAGSPLA AAAAAAAAER CSWLVPLVRT LLDRAYEPLG
1681LQWGLPSLPP TNGSPTFFED FQAFCATPEW RHFIDKQVQP TMSQFEMDTY AKSHDLMSGF
1741WNACYDMLMS SGQRRQWERA QSRRAFQELV LEPAQRRARL EGLRYTAVLK QQATQHSMAL
1801LHWGALWRQL ASPCGAWALR DTPIPRWKLS SAETYSRMRL KLVPNHHFDP HLEASALRDN
1861LGEVPLTPTE EASLPLAVTK EAKVSTPPEL LQEDQLGEDE LAELETPMEA AELDEQREKL
1921VLSAECQLVT VVAVVPGLLE VTTQNVYFYD GSTERVETEE GIGYDFRRPL AQLREVHLRR
1981FNLRRSALEL FFIDQANYFL NFPCKVGTTP VSSPSQTPRP QPGPIPPHTQ VRNQVYSWLL
2041RLRPPSQGYL SSRSPQEMLR ASGLTQKWVQ REISNFEYLM QLNTIAGRTY NDLSQYPVFP
2101WVLQDYVSPT LDLSNPAVFR DLSKPIGVVN PKHAQLVREK YESFEDPAGT IDKFHYGTHY
2161SNAAGVMHYL IRVEPFTSLH VQLQSGRFDC SDRQFHSVAA AWQARLESPA DVKELIPEFF
2221YFPDFLENQN GFDLGCLQLT NEKVGDVVLP PWASSPEDFI QQHRQALESE YVSAHLHEWI
2281DLIFGYKQRG PAAEEALNVF YYCTYEGAVD LDHVTDERER KALEGIISNF GQTPCQLLKE
2341PHPTRLSAEE AAHRLARLDT NSPSIFQHLD ELKAFFAEVV SDGVPLVLAL VPHRQPHSFI
2401TQGSPDLLVT VSASGLLGTH SWLPYDRNIS NYFSFSKDPT MGSHKTQRLL SGPWVPGSGV
2461SGQALAVAPD GKLLFSGGHW DGSLRVTALP RGKLLSQLSC HLDVVTCLAL DTCGIYLISG
2521SRDTTCMVWR LLHQGGLSVG LAPKPVQVLY GHGAAVSCVA ISTELDMAVS GSEDGTVIIH
2581TVRRGQFVAA LRPLGATFPG PIFHLALGSE GQIVVQSSAW ERPGAQVTYS LHLYSVNGKL
2641RASLPLAEQP TALTVTEDFV LLGTAQCALH ILQLNTLLPA APPLPMKVAI RSVAVTKERS
2701HVLVGLEDGK LIVVVAGQPS EVRSSQFARK LWRSSRRISQ VSSGETEYNP TEAR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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