LSBio
NAV1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NAV1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NAV1 antibodies →
$725.00each
Catalog Number: LS-G111133-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neuron navigator 1(NAV1) (NM_020443), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1213 | Neuron navigator 1 | NAV1 | POMFIL3 | Steerin-1 | STEERIN1 | Neuron navigator-1 | Unc-53 homolog 1 | UNC53H1 | KIAA1151
Origin Species
Human
NCBI GenBank
NM_020443

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
202.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGSSVKSVQ PEVELSSGGG DEGADEPRGA GRKAAAADGR GMLPKRAKAP GGGGGMAKAS
61AAELKVFKSG SVDSRVPGGP PASNLRKQKS LTNLSFLTDS EKKLQLYEPE WSDDMAKAPK
121GLGKVGSKGR EAPLMSKTLS KSEHSLFQAK GSPAGGAKTP LAPLAPNLGK PSRIPRGPYA
181EVKPLSKAPE AAVSEDGKSD DELLSSKAKA QKSSGPVPSA KGQEERAFLK VDPELVVTVL
241GDLEQLLFSQ MLDPESQRKR TVQNVLDLRQ NLEETMSSLR GSQVTHSSLE MTCYDSDDAN
301PRSVSSLSNR SSPLSWRYGQ SSPRLQAGDA PSVGGSCRSE GTPAWYMHGE RAHYSHTMPM
361RSPSKLSHIS RLELVESLDS DEVDLKSGYM SDSDLMGKTM TEDDDITTGW DESSSISSGL
421SDASDNLSSE EFNASSSLNS LPSTPTASRR NSTIVLRTDS EKRSLAESGL SWFSESEEKA
481PKKLEYDSGS LKMEPGTSKW RRERPESCDD SSKGGELKKP ISLGHPGSLK KGKTPPVAVT
541SPITHTAQSA LKVAGKPEGK ATDKGKLAVK NTGLQRSSSD AGRDRLSDAK KPPSGIARPS
601TSGSFGYKKP PPATGTATVM QTGGSATLSK IQKSSGIPVK PVNGRKTSLD VSNSAEPGFL
661APGARSNIQY RSLPRPAKSS SMSVTGGRGG PRPVSSSIDP SLLSTKQGGL TPSRLKEPTK
721VASGRTTPAP VNQTDREKEK AKAKAVALDS DNISLKSIGS PESTPKNQAS HPTATKLAEL
781PPTPLRATAK SFVKPPSLAN LDKVNSNSLD LPSSSDTTHA SKVPDLHATS SASGGPLPSC
841FTPSPAPILN INSASFSQGL ELMSGFSVPK ETRMYPKLSG LHRSMESLQM PMSLPSAFPS
901STPVPTPPAP PAAPTEEETE ELTWSGSPRA GQLDSNQRDR NTLPKKGLRY QLQSQEETKE
961RRHSHTIGGL PESDDQSELP SPPALPMSLS AKGQLTNIVS PTAATTPRIT RSNSIPTHEA
1021AFELYSGSQM GSTLSLAERP KGMIRSGSFR DPTDDVHGSV LSLASSASST YSSAEERMQS
1081EQIRKLRREL ESSQEKVATL TSQLSANANL VAAFEQSLVN MTSRLRHLAE TAEEKDTELL
1141DLRETIDFLK KKNSEAQAVI QGALNASETT PKELRIKRQN SSDSISSLNS ITSHSSIGSS
1201KDADAKKKKK KSWLRSSFNK AFSIKKGPKS ASSYSDIEEI ATPDSSAPSS PKLQHGSTET
1261ASPSIKSSTS SSVGTDVTEG PAHPAPHTRL FHANEEEEPE KKEVSELRSE LWEKEMKLTD
1321IRLEALNSAH QLDQLRETMH NMQLEVDLLK AENDRLKVAP GPSSGSTPGQ VPGSSALSSP
1381RRSLGLALTH SFGPSLADTD LSPMDGISTC GPKEEVTLRV VVRMPPQHII KGDLKQQEFF
1441LGCSKVSGKV DWKMLDEAVF QVFKDYISKM DPASTLGLST ESIHGYSISH VKRVLDAEPP
1501EMPPCRRGVN NISVSLKGLK EKCVDSLVFE TLIPKPMMQH YISLLLKHRR LVLSGPSGTG
1561KTYLTNRLAE YLVERSGREV TEGIVSTFNM HQQSCKDLQL YLSNLANQID RETGIGDVPL
1621VILLDDLSEA GSISELVNGA LTCKYHKCPY IIGTTNQPVK MTPNHGLHLS FRMLTFSNNV
1681EPANGFLVRY LRRKLVESDS DINANKEELL RVLDWVPKLW YHLHTFLEKH STSDFLIGPC
1741FFLSCPIGIE DFRTWFIDLW NNSIIPYLQE GAKDGIKVHG QKAAWEDPVE WVRDTLPWPS
1801AQQDQSKLYH LPPPTVGPHS IASPPEDRTV KDSTPSSLDS DPLMAMLLKL QEAANYIESP
1861DRETILDPNL QATL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1874 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.