Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NAT1 / AAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128063-100
Catalog Number: LS-G128063
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NAT1 (NM_001160175), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AAC1 | Arylamide acetylase 1 | MNAT | N-acetyltransferase type 1 | NATI | NAT-1 | NAT1
- Origin Species
- Human
- NCBI GenBank
- NM_000662
- SwissProt
- P18440
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLLLLHKEA ALEPQGPAVG YNSLPVSDDH HVSGIQARKK QQSVFWIKTE DQPTFNLLRK61GIMDIEAYLE RIGYKKSRNK LDLETLTDIL QHQIRAVPFE NLNIHCGDAM DLGLEAIFDQ121VVRRNRGGWC LQVNHLLYWA LTTIGFETTM LGGYVYSTPA KKYSTGMIHL LLQVTIDGRN181YIVDAGFGRS YQMWQPLELI SGKDQPQVPC VFRLTEENGF WYLDQIRREQ YIPNEEFLHS241DLLEDSKYRK IYSFTLKPRT IEDFESMNTY LQTSPSSVFT SKSFCSLQTP DGVHCLVGFT301LTHRRFNYKD NTDLIEFKTL SEEEIEKVLK NIFNISLQRK LVPKHGDRFF TITRTRPLEQKLISEEDLAANDILDYKDDDDKV
352 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.