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NARF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NARF Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120556-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NARF (NM_001038618), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NARF | IOP2
Origin Species
Human
NCBI GenBank
NM_012336
SwissProt
Q9UHQ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
44.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MTAEEGVQLS QQNAKDFFRV LNLNKKCDTS KHKVLVVSVC PQSLPYFAAK FNLSVTDASR
61RLCGFLKSLG VHYVFDTTIA ADFSILESQK EFVRRYRQHS EEERTLPMLT SACPGWVRYA
121ERVLGRPITA HLCTAKSPQQ VMGSLVKDYF ARQQNLSPEK IFHVIVAPCY DKKLEALQES
181LPPALHGSRG ADCVLTSGEI AQIMEQGDLS VRDAAVDTLF GDLKEDKVTR HDGASSDGHL
241AHIFRHAAKE LFNEDVEEVT YRALRNKDFQ EVTLEKNGEV VLRFAAAYGF RNIQNMILKL
301KKGKFPFHFV EVLACAGGCL NGRGQAQTPD GHADKALLRQ MEGIYADIPV RRPESSAHVQ
361ELYQEWLEGI NSPKAREVLH TTYQSQERGT HSLDIKW
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

397 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.