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NAP1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NAP1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NAP1L3 antibodies →
$504.00each
Catalog Number: LS-G116589-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NAP1L3 (NM_004538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BNAP | NAP1L3 | MB20
Origin Species
Human
NCBI GenBank
NM_004538
SwissProt
Q99457

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MAEADFKMVS EPVAHGVAEE EMASSTSDSG EESDSSSSSS STSDSSSSSS TSGSSSGSGS
61SSSSSGSTSS RSRLYRKKRV PEPSRRARRA PLGTNFVDRL PQAVRNRVQA LRNIQDECDK
121VDTLFLKAIH DLERKYAELN KPLYDRRFQI INAEYEPTEE ECEWNSEDEE FSSDEEVQDN
181TPSEMPPLEG EEEENPKENP EVKAEEKEVP KEIPEVKDEE KEVAKEITEV KAEEKADSKD
241CMEATPEVKE DPKEVPQVKA DDKEQPKATE AKARAAVRET HKRVPEERLQ DSVDLKRARK
301GKPKREDPKG IPDYWLIVLK NVDKLGPMIQ KYDEPILKFL SDVSLKFSKP GQPVSYTFEF
361HFLPNPYFRN EVLVKTYIIK AKPDHNDPFF SWGWEIEDCK GCKIDWRRGK DVTVTTTQSR
421TTATGEIEIQ PRVVPNASFF NFFSPPEIPM IGKLEPREDA ILDEDFEIGQ ILHDNVILKS
481IYYYTGEVNG TYYQFGKHYG NKKYRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

506 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.